肝硬化患者血清一氧化氮和几种白细胞介素检测的临床意义

Clinical significances of serum NO. IL-2、 IL-6、 IL-8 and sIL-2R in patients with hepatic cirrhosis

目的 探讨肝硬化患者血清一氧化氮 (NO)、白细胞介素 2 (IL 2 )、白细胞介素 6 (IL 6 )、白细胞介素 8(IL 8)及可溶性白细胞介素 2受体 (sIL 2R)变化的临床意义。方法 应用ELISA法测定肝硬化患者及正常对照组的血清IL 6、IL 8及sIL 2R含量 ;应用MTT法检测血IL 2活性 ;应用荧光法检测血清NO水平。结果 肝硬化患者血清IL 2、sIL 2R、IL 6、IL 8及NO水平 :(5 741.5 3± 4376 .5 2 )U/ml、(486 .76± 46 .41)U/ml、(15 .78± 3.0 4) pg/ml、(2 3.89± 2 .13)pg/ml及 (6 .33± 0 .37) μmol/L ,显著高于正常对照组 :(173.88± 92 .2 1)U/ml、(2 42 .36± 35 .78)U/ml、(6 .14± 3.12 ) pg/ml、(17.71± 1.32 )pg/ml及 (3.6 8± 0 .34 ) μmol/L ,并随肝功受损程度进行性增加。 结论 肝硬化患者血清IL 2、sIL 2R、IL 6及IL 8增加可能为NO增多的诱发因素 ;肝功能损伤可能是白细胞介素活性增加的重要原因。

Objective To investigate the changes and clinical significances of serum nitric oxide (NO), interleukin 2 (IL 2), interleukin 6 (IL 6), interleukin 8(IL 8) and soluble interleukin 2 receptor (sIL 2R) in patients with hepatic cirrhosis. Methods Serum IL 6, IL 8 and sIL 2R levels were determined by the double antibody sandwich ELISA method in cirrhotic patients and normal controls; Serum IL 2 activity was observed by MTT assay; Serum NO concentration was also measured by a flurometric assay. Results Concentration of serum IL 2, sIL 2R, IL 6, IL 8 and NO in cirrhotic patients were significantly higher than those in healthy volunteers. There was a close positive correlation between levels of serum interleukins and concentration of serum NO and the severity of liver dysfunction. Conclusions The excessive production of NO induced by interleukins is responsible for the hyperdynamic circulation in patients with cirrhosis.