HIV合并HBV/HCV感染患者外周血T细胞亚群的变迁

The changes of peripheral blood T lymphocyte subset in the patients of HIV concurrent infection with HBV/HCV

目的了解HIV合并HBV、HCV感染患者外周血T细胞亚群的变化特征,探讨HIV合并HBV和HCV感染后患者的免疫功能。方法采用三色流式细胞学检测技术,对26例HIV合并HBV和HCV感染患者(合并感染组)、35例单纯HIV感染患者(单纯感染组)和194例健康人群(健康对照组)外周血总T细胞、T4细胞、T8细胞含量进行测定并计算T4/T8比值。结果单纯感染组患者外周血总T细胞、T4细胞、T8细胞的百分含量和T4/T8比值分别为66.42±4.11、27.74±2.34、47.72±3.86和0.74±0.19,合并感染组分别为54.76±3.42、22.31±1.87、58.23±4.62和0.33±0.12,健康对照组值分别为69.98±5.79、35.25±5.16、25.08±4.34和1.45±0.28单纯感染组患者T4细胞和T4/T8比值明显低于健康对照组(P<0.05,P<0.01),T8细胞较对照组明显升高(P<0.05);合并感染组总T细胞、T4细胞和T4/T8比值明显低于健康对照组和单纯感染组(P<0.05,P<0.01),T8细胞较健康对照组和单纯感染组明显升高(P<0.05,P<0.01)。结论 HIV感染患者合并HBV和HCV感染将进一步加重体内免疫功能的紊乱;检测外周血T细胞亚群有利于HIV、HBV和HCV多重感染患者的鉴别诊断、病情监测以及预后判断,是一项十分有效的客观依据。

Objective To Analyze of the change character of peripheral blood T lymphocyte subset in the patients of HIV concurrent infection with HBV/HCV,and investigate the immune function of these patients.Methods Three color Flow cytometry was used to determine the peripheral blood total T cell,CD4＋T cell,CD8＋T cell and calculate CD4＋T/CD8＋T in 26 patients of HIV concurrent infection with HBV/HCV（concurrent infection group）,35 patients with HIV only（simple infection group） and 194 health people（control group）.Results The peripheral blood total T cell,CD4＋T cell,CD8＋T cell and CD4＋T/CD8＋T in simple infection group were 66.42±4.11,27.74±2.34,47.72±3.86 and 0.74±0.19,or concurrent infection group were 54.76±3.42,22.31±1.87,58.23±4.62 and 0.33±0.12;and control group were 69.98±5.79,35.25±5.16,25.08±4.34 and 1.45±0.28.Significantly higher percentage of CD8＋T lymphocyte,lower of CD4＋T lymphocyte and CD4＋/CD8＋ ratio in simple infection group were found,comparing to those in controls（P0.05 or P0.01）.It was also demonstrate that in the group of concurrent infection,the percentage of total T lymphocyte,CD4＋T lymphocyte and CD4＋/CD8＋ were lower and CD8＋T lymphocyte was higher than those in simple infection group and control group（P0.05 or P0.01）.Conclusion Concurrent infection with HBV and HCV in patient of HIV infection will aggravate immune disorder;Detection of peripheral blood T lymphocyte subset can be used to differential diagnosis,patients＇ condition motion and prognosis judgment in patients of HIV/HBV/HCV multiple infection.