脐血干细胞移植治疗肝硬化失代偿后T淋巴细胞亚群的观察

Alteration of T-lymphocyte subsets after treated by cord blood-derived stem cells in patients with decompensated cirrhosis

目的观察脐血干细胞移植对肝硬化失代偿患者免疫功能的影响。方法采用流式细胞术测定脐血干细胞治疗肝硬化失代偿前后患者总T细胞(CD3+)、T4细胞(CD3+CD4+)、T8细胞(CD3+CD8+)含量的变化。结果 51例肝硬化失代偿患者治疗前总T细胞、T4细胞、T8细胞含量及T4/T8比值分别为60.6±22.5、45.2±13.6、26.2±7.6和1.21±0.35,均明显低于健康对照组(P<0.05);脐血干细胞移植治疗1周后外周血总T细胞和T4细胞含量及T4/T8比值分别为68.5±17.6、51.2±14.6和1.71±0.65,均明显高于治疗前组(P<0.05);T8细胞含量21.3±8.5,明显低于治疗前组(P<0.05)。结论脐血干细胞治疗能有效改善肝硬化失代偿患者的免疫功能。

Objective To investigate the effect of cord blood-derived stem cells transplantation on immunologic function in patients with decompensated cirrhosis.Methods The content of T cell subsets of the patients with decompensated cirrhosis（DEC） who before and after treated with cord blood-derived stem cells transplantation was detected by flow cytometry.Results The study results shows that the concent of total T cells、T4 cells and T8 cells,the ratio of T4/T8 in decompensated cirrhosis was respectively 60.6±22.5,45.2±13.6,26.2±7.6,1.21±0.35,obviously higher than in control;After treated with cord blood-derived stem cells transplantation in DEC patients,the concent of total T ells、T4 cells and the ratio of T4/T8 were separately 68.5±17.6,51.2±14.6,1.71±0.65 and significantly higher than before treatment（P0.05）;the content of T8 cells was 21.3±8.5 and definitely lower than before treatment（P0.05）.Conclusion The transplantation of cord blood-derived stem cells on DEC patients can improve their function immunologic function significantly.