甘露聚糖肽对COPD患者淋巴细胞免疫活性的影响

The effect of Polyactin A on the lymphocyte immune-competence in patients with COPD

目的为甘露聚糖肽治疗慢性阻塞性肺病(COPD)的合理用药提供依据。方法对经流式细胞术确认淋巴细胞免疫功能低下的COPD患者,用不同浓度的甘露聚糖肽体外诱导淋巴细胞,采用3H-TdR与14CUR参入双标记法测定淋巴细胞参入计数值。结果在不同剂量的甘露聚糖肽的作用下,淋巴细胞参入计数和百万细胞参入计数值不同,在1mg/L时,3H-TdR和14CUR计数与对照瓶比较差异有统计学意义(P<0.05);当甘露聚糖肽浓度达到10mg/L时,3H-TdR和14CUR的参入计数值达到高点,与对照瓶比较差异有统计学意义(P<0.01);继续增加甘露聚糖肽至浓度50mg/L和100mg/L时,两项计数水平开始回落,所测数值与对照瓶比较差异无统计学意义(P>0.05),淋巴细胞参入率与上述两项计数的结果一致。结论甘露聚糖肽对淋巴细胞免疫功能有明显的调节作用,在较低剂量(1、10mg/L)时,有明显促进淋巴细胞DNA及RNA合成的效果,但具有双相性,表现出较强的剂量依赖性。

Objective To provide basis for reasonable medication and achieving desired effect in treating the COPD with Polyactin A.Methods Lymphocytes which selected by low immunologic function confirmed by flow cytometry were inducted by different density of Polyactin A.Then the double labeling way of 3H-TdR and 14C-UR were used to the lymphocyte counting value.Results Under function of different density Polyactin A,the interpolates counting values were different in the lymphocyte,the 3H-TdR and 14C-UR counting value also had statistics significance（P0.05） comparing the control bottle in 1 mg/L.Both of their value arrived at high point while Polyactin A was in 10 mg/L,and they had the extremely significance difference comparing the control bottle respectively（P0.01）.But continues increasing the density to 50 and 100 mg/L,the counting values started to descend,the values were not significant comparing the control bottle.The result of lymphocyte interpolation rate was as same as above.Conclusion Polyactin A has obvious control action to the immunologic function,and has obvious promote synthesis effect to lymphocyte DNA and RNA,but it has the double-phase and displayed strong dosage dependence.