减少样本数改良最小模型技术

Reduced sample number modified the minimal model

目的 为克服经典的最小模型技术 (MMT)样本数太多 (3 0个 )、历时太长 (3小时 )的缺陷 ,以减少样本数所获得的信息对估计参数和重建胰岛素曲线的误差降至最小范围。方法 试对经典 MMT进行样本数减至 12个和 10个、时间缩至 2小时改良 ,分别用 3 0、12和 10个样本三种方案的胰岛素改良 MMT(IMMMT)评估 16例葡萄糖耐量正常 (NGT)者和 13例 2型糖尿病病人的胰岛素敏感性 (SI)和葡萄糖效应 (SG)。结果 不仅 12个样本 ,而且 10个样本的 SI和 SG与 3 0个样本的 SI和 SG在每个组值都相近 ,相关性高 (r=0 74～ 0 92 ,P<0 0 0 0 1)。结论 提示样本数减至 10～ 12个、试验时间缩至 2小时改良 IMMMT是可行的。

Objective Classical minimal model technique (MMT) requires 30 samples and 3 hours. To overcome the defects that MMT needs so many the numbers of sample and time. Methods We chose a sample schedule that minimized the variance of the parameter estimates and the error in reconstructing the plasma insulin profile, trying to reduce the total number of samples of MMT from 30 to 12 or 10 and the time of MMT from 3 hours to 2 hours. Respectively using 30, 12 and 10 samples protocols, insulin sensitivity (S I) (S I30 , S I12 and S I10 , respectively) and glucose effectiveness (S G) (S G30 , S G12 and S G10 , respectively) were estimated in 16 subjects with normal glucose tolerance (NGT) and 13 patients with type 2 diabetes mellitus.Results There were strong relationships between not only S I12 and S I30 , but also S I10 and S I30 in every group (r=0 74～0 92,P<0 0001); S G12 and S G10 had highly relationships with S G30 in each group (r=0 86～0 93,P<0 0001).Conclusion The reformations reducing the total number of samples of MMT from 30 to 12 or 10 and the time of MMT from 3 hours to 2 hours may be feasible.