定量聚合酶链式反应技术检测溃疡创面纤维连接蛋白表达水平的变化

DETERMINATION OF FIBRONECTIN mRNA EXPRESSION IN PATIENTS WITH DIABETIC ULCERS BY QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE

目的 研究纤维连接蛋白在糖尿病患者足溃疡组织中的表达特征与规律,探讨纤维连接蛋白表达量的变化与糖尿病足溃疡发生的关系。方法 取患者溃疡创面及创缘组织为实验组,以患者正常皮肤为对照组,采用定量聚合酶链式反应(PCR) 方法检测纤维连接蛋白mRNA表达水平的变化。结果 在正常皮肤组织和溃疡组织中均存在纤维连接蛋白mRNA的表达,但其表达水平在溃疡组织中显著低于正常皮肤,表达量约为正常皮肤的34 % 。结论 糖尿病患者足溃疡组织中纤维连接蛋白mRNA表达水平降低。

Objective To determine the characteristics and regularity of fibronectin mRNA expression in diabetic ulcers, and to investigate the relationship between the changes of fibronectin mRNA expression and pathogenesis of diabetic ulcer. Methods Biopsies were removed from the margins of diabetic foot ulcers, included surrounding skin as experimental group, and the biopsies from normal skin of the same patients as control group. The mRNA expression of fibronectin was measured by quantitative RT PCR technique. Results The mRNA expression of fibronectin could be detected in both normal skin and diabetic foot ulcers, but the level of expression in diabetic ulcers was lower than that of normal skin. Conclusion The level of mRNA expression of fibronectin in diabetic ulcers is decreased, which suggest that the down regulation of transcription may be one of the mechanisms of chronic impaired ulcers.