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尿液流水线尿沉渣检验结果复检标准的探讨 被引量:5

Study the re-examination standards of the automatic urinalysis system for urine visible elements by microscope
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摘要 目的通过分析尿液流水线主要参数RBC,WBC的假阳性、假阴性、敏感性与特异性,探讨本实验室参考值,及显微镜复检规则。方法选取2 000例住院患者的晨尿标本进行分析,由AX-4280尿干化学分析仪、UF-1000i尿有形成分分析仪及Diasys显微镜3者组成流水线计数尿液有形成分,并进行统计学分析。结果 (1)UF-1000iRBC假阳性率10.78%,假阴性率0.00%,敏感性100.00%,特异性89.22%,符合率91.00%;WBC假阳性率13.10%,假阴性率0.00%,敏感性100.00%,特异性86.88%,符合率89.50%。(2)根据ROC曲线,确定UF-1000iRBC临界值为25个/微升,WBC临界值25个/微升;(3)通过分析,建立7条显微镜复检规则。结论尿液流水线大大提高检测速度,为提高检测准确性与精确性,必须建立本实验室的参考值及显微镜复检规则。 Objective To study the false positive,the false negative,the sensitivity and the specificity of the main parameters RBC,WBC of the urine line,determine the laboratory reference values,and at last establish the re-examination rules by microscope.Methods Selected the 2 000 cases of morning urine samples of patients hospitalized for statistics,calculated the amount of urine visible elements by AX-4280 urine dry chemistry analyzer,UF-1000i urine sediment analyzer and Diasys microscope,and analyzed statistically.Results(1)RBC false positive rate of the UF-1000i was 10.78%,false negative rate was 0.00%,sensitivity rate was 100.00%,specificity rate was 89.22%,and the precision rate was 91.00%;the WBC false positive rate of the UF-1000i was 13.1%,false negative rate was 0.00%,sensitivity rate was 100.00%,specificity rate was 86.88%,and the precision rate was 89.5%.(2)According to the ROC curve to determine the UF-1000i RBC reference value was≤ 25/μL,WBC was≤25/μL.(3)Through statistical analysis,seven microscopic re-examination rules were established.Conclusion UF-1000i automatic urinalysis system joint screening greatly improves the detection rat.For improving detection accuracy and precision,it should determine the laboratory reference values and establish the re-examination rules by microscope.
出处 《国际检验医学杂志》 CAS 2011年第21期2446-2447,2449,共3页 International Journal of Laboratory Medicine
关键词 参考值 尿液流水线 尿分析仪 显微镜复检规则 reference values the automatic urinalysis system urine dry chemistry analyzer the re-examination rules
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