类风湿关节炎患者血清转甲状腺素蛋白化学修饰的蛋白质组学分析

Proteomic analysis of chemically modified transthyretin in patients with rheumatoid arthritis

目的探讨血清转甲状腺素蛋白(TTR)水平的变化规律和不同的修饰类型在类风湿关节炎(RA)早期诊断中的临床价值。方法采用前瞻性开放研究,采用酶联免疫吸附试验(ELISA)测定RA早期、RA中、晚期和骨性关节炎(OA)患者及健康体检者(对照组)血清中TTR的含量;采用基质辅助激光解吸离子化飞行时间质谱(MALDI-TOF-MS)技术测定血清中TTR蛋白的化学修饰类型和比例。结果 RA早期患者血清中的TTR含量与对照组比较差异有统计学意义(t=4.57,P<0.05);TTR的修饰比例在RA早期组和中晚期组中各不相同,与文献报道相符,说明其随着病情的变化而变化。结论 RA患者早期血清中TTR含量的增高,TTR的修饰比例随着病情的变化而变化,均提示TTR可以作为RA早期诊断的潜在指标。

Objective To explore the changes of serum transthyretin（TTR） and the clinical value of different chemically modified TTR for the early diagnosis of rheumatoid arthritis（RA）.Methods Prospective opening research was performed.Serum levels of TTR of patients with early RA,medium and late RA or osteoarthritis（OA）,and healthy controls were determined by enzyme linked immunosorbent assay（ELISA）.Type and ratio of different chemically modified TTR were detected by matrix-assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF-MS）.Results Serum level of TTR in patients with early RA was higher than control group（t=4.57,P0.05）.Patients with early or medium and late RA were with different ratio of modified TTR.Conclusion Serum level of TTR in patients with early RA could increase and ratio of different modified TTR could change with the progress of disease condition,indicating TTR could be used for the early diagnosis of RA.