皮质酮复合丙泊酚对大鼠pCREB表达的影响

Effect of cortucisterone and propofol on pCREB expression among Wistar rats

目的探讨皮质酮复合丙泊酚对大鼠pCREB表达的影响。方法将24只21 d龄的Wistar大鼠随机分为4组(=6):对照组(Ⅰ组)、丙泊酚组(Ⅱ组)、皮质酮组(Ⅲ组)及丙泊酚+皮质酮组(Ⅳ组),分别以0.9%氯化钠注射液10 ml/kg、丙泊酚100 mg/kg、皮质酮10 mg/kg、丙泊酚100 mg/kg+皮质酮10 mg/kg腹腔注射,连续用药7d后,以免疫组织化学法检测前额叶皮质pCREB的表达。结果与Ⅰ组比较,Ⅱ组、Ⅲ组及Ⅳ组pCREB表达下调(均<0.05);与Ⅱ组、Ⅲ组比较,Ⅳ组pCREB表达减少(均<0.05)。结论 100 mg/kg丙泊酚或10 mg/kg皮质酮多次重复应用,大鼠pCREB的表达减少,两药复合应用时抑制作用增强。

Objective To investigate the effect of cortucisterone and propofol on pCREB expression among Wistar rats.Methods Thirty-two aged 21 days Wistar ats were randomly divided into four groups,with eight rats in each group.The control group（group Ⅰ） was injected with 0.9% sodium chloride injection,with the dose of 10 ml / kg;the propofol group（group Ⅱ） was injected with propofol,with dose of 100mg/kg;the cortucisterone group（group Ⅲ） was injected with cortucisterone,with the dose of 10mg/kg;the propofol and cortucisterone group（group Ⅳ）.Were injected with propofol（100 mg/kg） and cortucisterone（10 mg/kg）.Seven days later,pCREB expression was detected by immunohistochemical way.Results Compared to group I,expressions decreased in group Ⅱ,group Ⅲ and group Ⅳ（P0.05）.Compared to group Ⅱand group Ⅲ,expressions decreased in group Ⅳ（P0.05）.Conclusions Inhibition effects are strengthen by propofol combined with cortucisterone,with decrease of pCREB expressions.