碱性成纤维细胞生长因子结合静脉套接法修复周围神经缺损的实验研究

EXPERIMENTAL STUDY ON REPAIR OF PERIPHERAL NERVE DEFECT BY BASIC FIBROBLAST GROWTH FACTOR COMBINED WITH AUTOGENOUS VEIN GRAFT CONDUIT

目的 探讨碱性成纤维细胞生长因子(bFGF) 与静脉套接法修复神经缺损的作用。方法 新西兰大白兔54 只,分成三组,切断兔一侧坐骨神经,用自体静脉桥接。A组于静脉段内注入bFGF溶液0-2 ml( 浓度4 000 U/ml),B组注入等量的生理盐水,C组不注入任何物质。分别于术后10、30 及100 天取标本行HE 染色,光镜检查。其中100 天组先做传导速度测定,远端再生神经做髓鞘染色,轴突断面做图像分析并与健侧比较,计算恢复率。结果 各组在术后30 天均可见神经纤维通过静脉段,但以A 组较多,排列较齐。再生神经恢复率A 组比B组高,经统计学处理有显著性差异(P< 0.01)。

Objective To explore the effect of basic fibroblast growth factor (bFGF) combined with autogenous vein graft conduit on peripheral nerve regeneration. Methods Fifty four New Zealand rabbits were divided into three groups. The main trunk of sciatic nerve of rabbit in one side was severed and bridged by autogenous vein. 0.2 ml bFGF solution (4 000 U/ml) was intravenously injected to the vein graft conduit as group A, the same amount of saline solution as group B, and no solution injection as group C. Microscopic examination, axon video analysis and nerve conduct velocity were performed at the 10th, 30th, and 100th day after operation. Results The nerve fibers were grown into vein graft conduit in all groups at 30th after operation, they were more and regular in group A than that of group B and C, and the axon regeneration rate in group A was more than that of group B and C. Conclusion bFGF combined with autogenous vein graft conduit can markedly promote nerve regeneration.