青海青杨高效再生体系的建立

Establishment of an Efficient Regeneration System of Populus cathayana Rehd.var.Qinghai

以青海青杨茎段为外植体进行组织培养,研究了不同浓度激素对其再生的影响,建立了其高效的组培再生体系。结果表明:青海青杨在不定芽分化、试管苗伸长和增值以及试管苗生根的各阶段对培养基中激素的浓度反应较敏感,激素浓度低或高都会给试管苗生长带来很大影响。通过研究筛选,得到了青海青杨生长各阶段最佳的培养基:诱导茎段分化最佳的培养基为1/2MS+6-BA 0.5 mg.L-1+NAA 0.05 mg.L-1,其分化率82.0%;伸长及壮苗效果最好的培养基为MS+NAA0.005 mg.L-1;增殖效果最好的培养基为MS+6-BA 0.1 mg.L-1+NAA 0.3 mg.L-1;生根效果最好的培养基为1/2MS+IBA0.3 mg.L-1。

The shoots of Populus cathayana Rehd. var. Qinghai were used as explants for tissue culture to study the effects of different hormone concentrations on the plant＇ s regeneration system and a high-efficient tissue culture regeneration system of P. cathayana Rehd. var. Qinghai was established. It was found that P. cathayana Rehd. var. Qinghai was very sensitive to hormone concentration. The concentration of hormone had great impact on their growth in various stages. The best culture medium for induction and differentiation of P. cathayana Rehd. var. Qinghat was 1/2MS ＋6-BA 0.5 mg · L^-1 ＋NAA 0.05 mg · L^-1, on which the differentiation rate was 82.0% ; The best cuhu.re medium for bud growth was MS ＋ NAA 0.005mg · L^-1; The best culture medium for proliferation was MS ＋ 6- BA 0.1mg · L^-1 ＋ NAA 0.3 mg · L^-1; The best culture medium for rooting was 1/2MS ＋ IBA0.3 mg · L^-1.