摘要
目的:观察CDK11p58在H2O2诱导的PC12细胞凋亡中的表达及机制。方法:以H2O2处理PC12细胞和(或)过表达CDK11p58及其激酶突变的载体后,蛋白质免疫印迹观察CDK11p58的表达,流式细胞分析术分析PC12细胞的凋亡。结果:将CDK11p58表达载体转入PC12细胞中,在予H2O2处理细胞后,过表达CDK11p58的PC12细胞凋亡较未过表达明显增加,差异有统计学意义(P<0.05)。在转染双突变CDK11p58表达质粒PC12细胞中,H2O2诱导细胞凋亡较对照组无增高,但较转染全长的CDK11p58的表达质粒的细胞组有显著降低,差异有统计学意义(P<0.05)。结论:CDK11p58可以促进H2O2诱导的PC12细胞的凋亡。
Objective:To investigate the role of CDK11p58 in hydrogen peroxide induced PC12 cells apoptosis.Methods:PC12 cells were treated with H2O2 and/transfected with CDK11 expression and kinase site mutant expression vector for 24 hours,the expression of CDK11p58 were deteceted by western blot,and the cell apoptosis was detected by flow cytometry.Results:H2O2 could induced CDK11p58expression and its kinase activities were essential in the process.Conclusion:CDK11p58 can promote H2O2 induced PC12 cells apoptosis.
出处
《交通医学》
2011年第6期549-551,共3页
Medical Journal of Communications
基金
江苏省高校自然科学基金资助项目(09KJD310005)
南通市社会科学发展计划资助项目(S2008020)