CDK11p58在H_2O_2诱导PC12细胞凋亡中表达及意义

The effect of CDK11p58 in hydrogen peroxide induced PC12 cells apoptosis

目的:观察CDK11p58在H2O2诱导的PC12细胞凋亡中的表达及机制。方法:以H2O2处理PC12细胞和(或)过表达CDK11p58及其激酶突变的载体后,蛋白质免疫印迹观察CDK11p58的表达,流式细胞分析术分析PC12细胞的凋亡。结果:将CDK11p58表达载体转入PC12细胞中,在予H2O2处理细胞后,过表达CDK11p58的PC12细胞凋亡较未过表达明显增加,差异有统计学意义(P<0.05)。在转染双突变CDK11p58表达质粒PC12细胞中,H2O2诱导细胞凋亡较对照组无增高,但较转染全长的CDK11p58的表达质粒的细胞组有显著降低,差异有统计学意义(P<0.05)。结论:CDK11p58可以促进H2O2诱导的PC12细胞的凋亡。

Objective：To investigate the role of CDK11p58 in hydrogen peroxide induced PC12 cells apoptosis.Methods：PC12 cells were treated with H2O2 and/transfected with CDK11 expression and kinase site mutant expression vector for 24 hours,the expression of CDK11p58 were deteceted by western blot,and the cell apoptosis was detected by flow cytometry.Results：H2O2 could induced CDK11p58expression and its kinase activities were essential in the process.Conclusion：CDK11p58 can promote H2O2 induced PC12 cells apoptosis.