传染性单核细胞增多症患儿外周血Th17细胞表达的研究

Preliminary study on Th17 cells in peripheral blood of child patients with infectious mononucleosis

目的探讨传染性单核细胞增多症(IM)患儿外周血Th17细胞、IL-17、IL-6水平的变化及临床意义。方法分离IM患儿和健康者外周血单个核细胞(PBMC),免疫磁珠阴选CD4+T淋巴细胞,然后加佛波酯/离子霉素(PMA/Ion),经固定,透膜处理进行细胞内染色,流式细胞术检测CD4+T淋巴细胞内IL-17、IL-6水平。结果 IM患儿急性期IL-17、IL-6表达水平显著高于恢复期(P<0.01),两者又显著高于健康对照组(P<0.01)。结论 IM患儿外周血中存在异常增高的Th17细胞及其分泌的细胞因子,且其水平与病情活动相关,是判断IM患儿免疫状态和预后的指标之一。

Objective To study the changes and clinic significance of Th17 cells of IL-17,IL-6 in peripheral blood of child patients with infectious mononucleosis（IM）.Methods CD4＋ T lymphocytes were negatively selected from peripheral blood mononuclear cells（PBMC）,separated from peripheral blood of child patients with IM and healthy controls,and were analyzed for the expression level of IL-17 and IL-16 by flow cytometry,after being treated with PMA/Ion,fixation and intra-cellular dyeing by membrane penetration.Results The expression levels of IL-17 and IL-6 in child patients with IM at acute stage were higher than at convalescence stage（P0.01）,and all of them were higher healthy controls（P0.01）.Conclusion There could be abnormal increase of Th17 cells and related cytokines in child patients with IM,both of which might be correlated with pathogenetic condition and could be performed as indexes for the estimation of immune function and prognosis of child patients with IM.