楠竹叶乙醇提取液与水煮液的抑菌效果比较

The compare on the antibacterial effect of bamboo leaves ethanol extracts and bamboo leaves decoction

目的比较楠竹叶无水乙醇提取液与楠竹叶水煮液的抑菌效果。方法采取琼脂平板扩散法测定楠竹叶无水乙醇提取液与楠竹叶水煮液的抑菌效果及其最小抑菌浓度(MIC)。结果无水乙醇提取液与水煮液对金黄色葡萄球菌、表皮葡萄球菌、大肠杆菌、伤寒杆菌、普通变形杆菌、枯草芽孢杆菌均有明显的抑菌效果,当提取液的浓度在100%和50%时的最小抑菌浓度分别为6.25%、12.5%、3.13%、6.25%、12.5%、25%和12.5%、12.5%、25%、12.5%、6.25%、50%。结论无水乙醇提取液的抑菌效果与浓度有密切关系,而水煮液两种浓度的抑菌效果与浓度影响不大。且提取液浓度在50%时与水煮液浓度在100%时的抑菌效果差距不大。因此,楠竹叶的水煮液对G+和G-菌的感染均有辅助疗效的作用。

Objective To compare on the antibacterial effect of bamboo leaves ethanol extracts and bamboo leaves decoction.Methods Agar plate diffusion method was used to determine ethanol extract of bamboo leaves and bamboo leaves and decoction antibacterial effect of the minimum inhibitory concentration.Results It showed that ethanol and decoction extract were significant antibacterial effect against Staphylococcus aureus,Staph.epidermidis,Escherichia coli,Salmonella typhi,proteus vulgaris,Bacillus subtilis.the minimal inhibitory concentration of the extract for them were 6.25%,12.5%,3.13%,6.25%,12.5%,25% and 12.5%,12.5%,25%,12.5%,6.25%,50% when the concentration of extract at 100% and 50%.Conclusion The experiment indicates that the antibacterial effect of bamboo leaves ethanol extracts is closely related to the concentration,and the antibacterial effect of bamboo leaves decoction is little related to the concentration,and the antibacterial effect of extract concentration in 50% and decoction concentration in 100% are not differ much.Therefore,the decoction of bamboo leaves have secondary treatment effects for gram positive and negative bacteria.