艾滋病患者粪便隐孢子虫染色技术探讨

Research on staining technology for the examination of cryptosporidium in the feces of patients with AIDS

目的选择一种简便的方法检测隐孢子虫,为艾滋病患者机会性感染引起隐孢子虫病的诊断提供实验室依据。方法收集253份艾滋病患者粪便标本,采用甲醛-乙酸乙酯沉淀浓集菌加改良抗酸染色法检测隐孢子虫。结果 32份粪便标本隐孢子虫阳性,253份艾滋病患者标本合并隐孢子虫阳性率12.65%。结论甲醛-乙酸乙酯沉淀浓集菌加改良抗酸染色法染色效果佳,对艾滋病患者粪便中隐孢子虫的检测敏感,适合于基层医院推广使用。

Objective To explore the staining technology for the examination of cryptosporidium in order to provide laboratory references for the diagnosis of cryptosporidiosis caused by opportunistic infection in AIDS patients.Methods Feces samples of 253 cases of AIDS patients were collected and determined for cryptosporidium by enrich with formalin-ethyl acetate sedimentation and modified acid-fast staining.Results 32 samples were detected with positive result,and the positive rate in the 253 cases of AIDS patients was 12.65%.Conclusion Staining technology,applying formalin-ethyl acetate sedimentation and modified acid-fast staining were more sensitive and efficient,which could be suitable for the application in elementary hospitals.