同种异体骨基质明胶在椎体间融合术中的实验研究

EXPERIMENTAL STUDY ON APPLICATION OF ALLOGENEIC BONE MATRIX GELATIN IN THE INTERVERTEBRAL FUSION

目的 通过同种异体骨基质明胶(BMG) 在腰椎间诱导成骨的实验结果,证明同种BMG 能够在椎间诱导成骨,达到椎间融合的目的。方法 以兔腰椎间盘摘除后的椎间隙作为动物模型,分别植入明胶海绵、同种BMG、脱钙骨基质和酒精保存骨。植入后4 、8 及12 周进行X 线摄片和组织学检查。结果 同种BMG 无明显免疫排斥反应,并且具有良好的骨诱导作用,术后4 及8 周有大量新骨出现于椎间隙中央部,术后12 周椎间完全融合。结论 同种BMG 通过骨诱导作用在椎体间成骨融合。

Objective To testify the inductive osteogenesis of allogeneic bone matrix gelatin (BMG) in promoting intervertebral fusion. Methods The gelatin sponge, allogeneic BMG, decalcified bone matrix (DBM) and alcohol conserved bone were implanted respectively into the intervertebral space of rabbit, whose intervertebral discs were removed before implantation. The intervertebral spaces were evaluated by X ray and histological examination at 4, 8, and 12 weeks after operation. Results No obvious immune rejection was observed. Amounts of new bone were formed in the intervertebral spaces at 4 and 8 weeks. And complete infusion of the intervertebral spaces were appeared at 12 weeks. Conclusion Allogeneic BMG can promote bone fusion of intervertebral spaces through osteoinduction, which suggests that allogeneic BMP is an ideal substitute for bone replacement.