人胎儿膀胱发育中肥大细胞的组织化学研究

HISTOCHEMICAL STUDY ON MAST CELLS IN THE DEVELOPING BLADDER OF HUMAN FETUS

目的：研究人胎儿膀胱的肥大细胞的组织化学性质的变化。材料和方法：取４３例不同胎龄的人胎儿膀胱切片做ＨＥ染色和ＡＢ－Ｓ染色并测定肥大细胞的临界电解质浓度值及硫酸小蘖硷荧光染色。结果：６月龄前肥大细胞数量少，其颗粒经 ＴＢ染色呈淡紫蓝色，ＡＢ－Ｓ染色呈蓝色，临界电解质浓度值偏低，其颗粒的糖胺多糖的硫酸化程度偏低；６月龄后肥大细胞数量则明显增多，颗粒经ＴＢ染色呈深紫红色，ＡＢ－Ｓ染色出现红色及红蓝混合色，临界电解质浓度值偏高，颗粒的糖胺多糖的硫酸化程度也偏高，可合肝素。结论：在人胎儿膀胱发育中肥大细胞的组化性质发生了明显改变。

To study the histochemical property changes of the mast cells in bladder of human fetus. Materials and Methods: The sections of different months old fetuses were stained respectively with Toluidine blue(TB) and Alcian blue-Safranin(AB-S) mixture. The critical electrolyte concentration (CEC) in the mast cells and their fluorescent characteristic of Berberine sulphate staining were also investigated. Results: In fetuses younger than 6 months, the number of mast cells was less and the granules were pale violet and blue with TB and AB-S stainning respectivey, the concentration of CEC was also lower and their granular glycosaminoglycan had a lower degree of sulphation. However, in fetuses older than 6 months the number of mast cells increased greatly, the granules stained with TB showed deep violet color and red or mixed red-blue in AB-S mixture. The results showed a positive relationship between critical electrolyte concentration and high sulphation of granular glycosaminoglycan. Conculsion: Significant changes takes place in the histochemical properties of the mast cells during the development of the bladders of human fetus.