肺癌患者肺泡巨噬细胞表现HLA-DR的表达

Expression of HLA DR on Alveolar Macrophages from Patients with Lung Cancer

【目的】了解肺癌患者肺泡巨噬细胞表面人白细胞抗原DR(HLADR)的表达。【方法】经支气管肺泡灌洗获肺泡巨噬细胞,贴壁分离及培养,免疫组化方法检测HLADR表达的阳性细胞百分率。【结果】①肺癌及良性肺病患者,其肺泡巨噬细胞未经刺激,均已有部分表达HLADR,但前者明显高于后者;②干扰素α(IFNα)或脂多糖(LPS)刺激后,HLADR的表达均增加,且两者联合刺激优于单独刺激;③无论刺激与否,肺癌组荷瘤侧肺与非荷瘤侧肺比较,肺泡巨噬细胞表达HLADR的阳性细胞率明显增高。【结论】肺癌患者,尤其是肿瘤局部,肺泡巨噬细胞可能通过表达HLADR提高抗原递呈功能。IFNα、LPS均能明显增强肺癌患者肺泡巨噬细胞的抗原提呈功能,且两者有协同作用。

Objective To observe the expression of human leukocyte antigen DR (HLA DR) on alveolar macrophages. Methods The alveolar macrophages obtained by bronchoalveolar lavage, were separated and incubated. The expression of HLA DR was detected and the positive rates of alveolar macrophages were compared. Results ①Alveolar macrophages from all cases express HLA DR without stimulation. The positive rate of alveolar macrophages was higher from patients with lung cancer than that from cases with nonmalignant pulmonary diseases. ② Expression of HLA DR in case after stimulation with IFN α or LPS were higher than that wihtout stimulation, and stimulation with IFN α and LPS in combination was the highest. ③Whether stimulated or not, the positive rate of alveolar macrophages from the tumor bearing side of lung was higher than that from the nontumor bearing side of lung. Conclusion The funtion that alveolar macrophages submit antigen was enganced, because the alveolar macrophages aroud the tumor express HLA DR. The function that alveolar macrophages submit antigen was enhanced after stimulated with IFN α or/and LPS, and the effection of IFN α and LPS was cooperative.