RP-HPLC法测定橘红丸中甘草苷、柚皮苷、橙皮苷和迷迭香酸

Determination of liquiritin,naringin,hesperidin,and rosmarinic acid in Juhong Pill by RP-HPLC

目的建立同时检测橘红丸(化橘红、陈皮、甘草、炒紫苏子等)中甘草苷、柚皮苷、橙皮苷和迷迭香酸的测定方法。方法采用RP-HPLC法。C18色谱柱;甲醇-0.3%磷酸水溶液为流动相;体积流量1.0 mL/min;检测波长284 nm。结果根据回归方程,4种成分线性范围分别为甘草苷2.16～64.8μg/mL,R2=0.999 6;柚皮苷7.59～227.7μg/mL,R2=0.999 2;橙皮苷7.54～226.62μg/mL,R2=0.999 5;迷迭香酸1.704～51.12μg/mL,R2=0.998 9。平均回收率分别为甘草苷102.1%,RSD 1.43%(n=6),柚皮苷100.8%,RSD 0.71%(n=6),橙皮苷98.7%,RSD 1.44%(n=6),迷迭香酸99.6%,RSD 0.82%(n=6)。结论该方法经济适用,操作简便、准确,重复性好,可用于橘红丸的质量控制。

AIM To develop a method for determining liquiritin, naringin, hesperidin, and rosmarinic acid in Juhong Pill （ Cirri grandis Exocarpium, Cirri reticulatae Pericarpium, Glycyrrhizae Radix et Rhizoma, Perillae Fructus, etc. ）. METHODS The quantitative analysis was carried out on a column of Thermo Hypersil BDS Cls by RP-HPLC, by using a mobile phase of CHaOH -0.3% phosphoric acid under a flow rate of 1.0 mL/min. 284 nm was selected as the detection wavelength. RESULTS The linear ranges of liquiritin, naringin, hesperidin, and rosmarinic acid fell within the ranges of 2.16-64.8 I^g/mL,7.59-227.7 tzg/mL ,7. 54-226. 62 tzg/mL and 1. 704- 51.12 ~zg/mL, respectively. The four components showed good linear correlations （ R2 = 0. 999 6, 0. 999 2, 0. 999 5, 0. 998 9）. The recoveries were 102.1% for liquiritin, 100.8% for naringin, 98.7% for hesperidin, 99.6% for rosmarinic acid, respectively. The relative standard deviations were 1.43%, 0.71%, 1.44%, and 0.82%, respectively （n =6）. CONCLUSION This method is simple, economical, accurate, reproducible and convenient for the quality control over Juhong Pill.