仔猪水肿病大肠杆菌的分离鉴定及其毒力基因的检测

Identification of Escherichia coli Inducing Edema Disease of Pig and Detection of Virulence Genes

为了确定新疆石河子地区部分猪场断奶仔猪发病及死亡原因,本研究以疑似仔猪水肿病的病料为研究对象,从中分离得到3株细菌,通过形态特点、培养特性、生化特性等观察确定为大肠杆菌。3株分离菌血清型均为O139,且均能使人工感染小鼠死亡。药敏实验结果表明,分离株对部分抗生素有不同程度的耐药性。PCR检测结果显示,3株菌均携带黏附素(FedA)和类志贺毒素(SLT-2e)基因。由此可以确定可离的3株细菌是本次仔猪感染死亡的病原。

Three bacteria were isolated from edema disease in piglets in some farms from Shihezi,Xinjiang.Those isolates were comfirmerd to be E.coli through morphological characteristic,culture characteristic,biochemical characteristic.They were serotype O139 and caused the death of mice in artificial experiment.The results of drug sensitivity test indicated that the 3 isolates had resistance to some antibiotic in varying degrees.The 3 isolates had virulence genes of FedA and SLT-2e with PCR.The 3 isolates were pathogen inducing edema disease of piglets.