层粘连蛋白对金属蛋白酶抑制剂-2基因转染人胃癌细胞转移相关行为影响的研究

Study of influence of laminin on the biological behavior of human gastric cancer cells transfected with TIMP-2 gene

目的 探讨层粘连蛋白对组织金属蛋白酶抑制剂 2 (Tissueinhibitorofmetalloproteinase 2 ,TIMP 2 )基因转染胃癌细胞的生物学行为变化。方法 采用基因转染的方法将人全长TIMP 2cDNA基因转染入人胃癌SGC 790 1细胞系中 ,利用层粘连蛋白对肿瘤细胞的诱导迁移作用 ,在体外侵袭模型中观察转染基因细胞侵袭能力的改变。结果 转染TIMP 2基因的SGC 790 1细胞侵袭能力 (33.1± 11.3细胞 /h)明显低于未转染组 (135 .7± 2 1.6细胞 /h ,P <0 .0 1) ,细胞的粘附能力受到抑制 ,转染组(2 36 .7± 37.3细胞 /h)低于未转染组 (6 2 6 .1± 45 .1细胞 /h)。且细胞的增殖能力未发生明显变化。结论 TIMP 2基因可阻抑层粘连蛋白对瘤细胞的诱导侵袭影响 ,参与瘤细胞转移的阻断机制。

Objective To study the changes of biological behavior of human gastric cancer cells SGC 7901 transfected with tissue inhibitor of metalloproteinase 2 (TIMP 2) gene by Laminin. Methods TIMP 2 gene was transfected into human gastric cancer cells SGC 7901 by Liprofectmin technique. A model of tumor invasion in vitro was used to determine the malignant phenotype of tumor cells. The transfected and untransfected groups were determined during experimental period include Laminin attachment, spreading, migration and proliferation of SGC 7901 cells. Results The invasion ability in SGC 7901 T2 (33.1±11.3 cells/h) was significantly lower than that in the controls (135.7±21.6 cells/h, P< 0.01). TIMP 2 could inhibit Laminin attachment, spreading, migration and proliferation of SGC 7901 cells. Conclusions The inhibitory effects of TIMP 2 on cancer metastasis is related to Laminin. It plays a key role in blockage of cancer metastasis.