氧化砷诱导胃癌细胞凋亡的实验研究

Induction of apoptosis by arsenic trioxide on gastric cancer cell lines

目的 研究三氧二化砷 (氧化砷 )对胃癌细胞的诱导凋亡作用。方法 应用TUNEL染色、流式细胞仪技术研究氧化砷对胃癌细胞MKN 45、MKN 2 8的诱导凋亡作用。结果 氧化砷作用于不同分化程度的胃癌细胞后 ,可看到较为典型的细胞凋亡的形态学变化 :细胞核固缩 ,染色质凝集 ,呈新月型紧贴于核膜周边 ,核碎裂 ,染色质片断化 ,凋亡小体形成等。流式细胞仪DNA直方图上出现典型的亚二倍体的“凋亡峰”。TUNEL染色法显示 ,细胞凋亡指数在 1.4%～ 9.5 %之间。结论 氧化砷对胃癌细胞具有诱导细胞凋亡的作用 ,具有治疗胃癌的潜在价值 ,值得进一步研究。

Objective To study the effects of arsenic trioxide (As 2O 3) on gastric cancer cells in induction of apoptosis.Methods Apoptosis and cell cycle changes of MKN 45 and MKN 28 induced by As 2O 3 were investigated by TUNEL method and flow cytometry.Results After 12 hours of exposure to the drug, MKN 45 and MKN 28 exhibited morphologic features of apoptosis, including cell shrinkage, nuclear condensation, DNA fragmentation and formation of apototic bodies. A typical subdiploid peak before G 0/G 1 phase was observed by flow cytometry. The apoptic index was 7-15% by TUNEL and FACS assay.Conclusions Arsenic trioxide can induce apoptosis of gastric cancer cells and it might have a promising prospect in the treatment of gastric cancer, and worth further studies.