芪丹益肾冲剂对大鼠原位免疫复合物性肾炎的作用

Therapeutic effects of the Qidanyishen instant granules on situ immune complex nephritis in rats

[目的]观察复方芪丹益肾冲剂对阳离子化牛血清白蛋白诱导的大鼠原位免疫复合物性肾炎的治疗作用.[方法]将Wistar大鼠随机分为正常对照组、模型对照组、芪丹益肾冲剂大、小剂量组及肾炎舒组.除正常对照组外其他4个组大鼠通过注射给予阳离子化牛血清白蛋白建立原位免疫复合物性肾炎模型.建立模型第3周开始给药,建立模型第6周末处死大鼠,采血,取肾脏,测定各项生物化学指标,观察形态学改变.[结果]芪丹益肾冲剂大、小剂量组尿蛋白定量与模型对照组比较差异均具有统计学意义(P<0.01).血清白蛋白、总胆固醇、三酰甘油、血肌酐及尿素氮水平与模型对照组比较差异均有统计学意义(P<0.05,P<0.01).芪丹益肾冲剂可减轻原位免疫复合物性肾炎大鼠基底膜增厚,改善电子致密物沉积情况.[结论]芪丹益肾冲剂可降低原位免疫复合物性肾炎模型大鼠的24h尿蛋白定量,改善肾功能,降低血脂水平,减轻肾小球病理改变.

OBJECTIVE To observe the therapeutic effects of Qidanyishen instant granules on the membranous glomerulonephritis induced by cationized bovine serum albumin（C-BSA） in rats.METHODS Wistar rats were randomly divided into 5 groups,including normal control group,model group,Qidanyishen instant granules large and small dosage groups and Shenyanshu group.Except normal control group,other 4 groups were injected with C-BSA to establish in situ immune complex nephritis.After establishment of nephritis model at 3 weeks,drugs were administered,and all animals were sacrificed at 6 weeks,and the blood and kidney specimen were collected to measure serum biochemical tests and observe histomorphologic changes.RESULTS As compared with the model group,urinary protein were reduced significantly（P0.01） and serum albumin,TC,TG,Scr and BUN were also decreased significantly in both large and small dosage groups of Qidanyishen instant granules（P0.05,P0.01）.The Qidanyishen instant granules reduced the thickness of basement membrane and lessen electron dense deposit in situ immune complex nephritis in rats.CONCLUSION The Qidanyishen instant granules can reduce proteinuria,improves the renal function,decreases lipidemia,and alleviates pathologic damage in situ immune complex nephritis induced by C-BSA in rats.