泌尿生殖道解脲支原体合并人型支原体感染耐药性分析

Analysis on the drug -resistance of antimicrobial susceptibility tests for Uu combined with MH infection

目的了解本地区泌尿生殖道解脲支原体(Uu)对9种抗菌药的敏感性及Uu合并人型支原体(MH)感染后的耐药性变化。方法支原体鉴定及药敏采用体外液体培养法。试剂盒由珠海丽珠生物公司提供。实验步骤按试剂盒操作手册进行。结果检测出Uu单独感染阳性病例33例,UU合并MH感染病例27例。Uu合并MH感染组对克拉霉素、阿奇霉素、罗红霉素耐药率较Uu单独感染组的耐药率显著性增加(P<0.05);而对强力霉素、美满霉素、交沙霉素、氧氟沙星、左氧沙星司帕沙星的耐药率两组问无显著性变化(P>0.05)。结论对Uu合并MH感染者,应首选美满霉素和强力霉素,选择应用左氧氟沙星、氧氟沙星、司帕沙星、交沙霉素;而克拉霉素、阿奇霉素、罗红霉素则不宜选用。

Objective To investigate the sensitivity of Uu to 9 antimicrobial drugs and the change of drug-resistance on the infection of Uu combined with MH in this region. Methods The identification and antimicrobial susceptibility of Mycoplasma was done by the method of liquid culture in vitro. Kits were provided by Zhuhai Lizhu biological company. The experimental procedures were carried out by kit manual. Results 33 cases were examined with the infection of Uu, 27 cases were examined with the infection of Uu combined with MH. The rate of drug-resistance to clarithromycin, azithromycin, roxithromycin for the cases of Uu combined with MH infection was obviously higher than the cases of Uu infection only （P〈0.05）, and there was not remarkably difference for the drug-resistance to doxycycline, minocycline, josamycin, ofloxacin, levofloxacin and sparfloxacin in the two group cases （P〉0.05）. Conclusion For the cases infected with Uu and MH, minocycline and doxycycline should be taken as the first choice, levofloxacin, ofloxacin, sparfloxacin and josamycin should be considered selectively, and clarithromycin, azithromycin and roxithromycin should not be optioned.