响应曲面法提取苦白蹄中总三萜成分

Extracting total triterpenes from Fomes officinalis ames with response surface methodology

目的采用响应对苦白蹄中总三萜类成分进行提取。方法在单因素试验基础上,利用响应曲面法中心组合设计,采用超声波辅助萃取法对苦白蹄中总三萜提取工艺参数进行优化分析。选择超声频率、超声时间和超声温度作为优化因素,研究不同因素水平对苦白蹄总三萜得率的影响。结果通过响应面分析得到总三萜提取的最优条件:超声频率70 Hz、提取时间35 min、提取温度40℃。结论总三萜类化合物提取的实测结果与响应曲面拟合所得方程的预测值符合良好。

Objective Total triterpenes were extracted from Fomes officinalis ames with response surface method. Methods The ultrasonic-assisted extraction process of total triterpenoids from Fomes officinalis ames was studied. Based on the experiment of singIe factor, the mathematical regression model was established about the dependent varia- ble （extraction yield of triterpenes） and independent variables （ Ultrasonic frequency, extraction time, extraction temperature） through Box-Benhnken center composite design and response surface methodology. Results The optimum extraction conditions were as follow：Ultrasonic frequency was 70 Hz, extraction time was 35 min, extraction tempera- ture was 40℃. Conclusion Total triterpenoids extract experiment proved that there are good fitness between the predicted and actual value.