HPLC法测定人血清中氨甲喋呤血药浓度

Determination of MTX in human serum by HPLC with UV Detector

目的：建立反相高效液相色谱法测定血清中氨甲喋呤浓度的方法。方法：以甲醇∶醋酸盐缓冲液为流动相，茶碱作内标，检测波长为３０６ｎｍ。结果：在０～８μｇ·ｍｌ－１范围内呈良好线性关系，ｒ＝０．９９９９。日内及日间测得ＲＳＤ分别为３．６６％，４．０３％。平均回收率为９５．７％，血清中最低检测浓度为５０ｎｇ·ｍｌ－１。结论：本法具有快速、准确，操作简便，流动相成本低等优点。

Aim To establish a HPLC method for the Determination of MTX in human serum.Methods Theophyline was selected as internal standard.The UV detector was set at 306 nm.The mobil phase consisted of methanol and 0.05 M HAC-NaAC buffur (30:100).Results the detection limit was 50ng·ml -1 .The good linearity was from 0 to 8 (g·ml -1 .The average recovery was 95.7%.Within day and between days were 3.66% and 4.03% respectly. Conclusion this method is sensitive,rapid accurate,inexpensive enough to be used for clinical therapeutic drug monitoring.