MgSO_4对大鼠脑缺血-再灌注损伤的保护作用

The protection of MgS_4, on Wistar rats of cerebral ischemia-reperfusion injury

目的验证MgSO4作为NMDA受体非竞争性拮抗剂对大鼠脑缺血再灌注后的保护作用及其与凋亡的关系。方法：用线栓法制作Wistar大鼠大脑中动脉栓塞模型．术后2小时予以再通．分别于再通的同时及再通后2小时给予不同剂量MgSO4治疗。术后21小时进行运动功能评估．测定脑组织含水量，观察病理组织形念学变化．并计算每视野下凋亡细胞数量。结果：早期大剂MgSO4组大鼠运动功能改善明显．并可减轻脑组织含水量．但早期给予MgSO4在短期内也不能减少凋亡细胞数目。结论：MgSO4作为兴奋性氨基酸受体（EAAs-R）非竞争性拮抗剂对大鼠脑缺血-再灌注有保护作用。

Objective To investigate the protective effects of MgSO4, on cerebral reperfusion injury.Methods The animal models of reformed middle cerebral artery occlusion (MCAO) ) were made in 72 oldWistar rats, which were treated by MgSO4: in different doses at varying stages. 21 hours after operation,the motive function was evaluated according to 0～ 5 grades, then all the rats were killed to determinecerebral tissue contents of water, to observe pathological changes and measure the number of apoptic cells.Results The motive function in rats were reIatively improved after given higher dose of MgSO4 at earlierstage; MgSO4, can ameliorate brain edema; Compared with contrl group, MgSO4, no exhibit a certain role inreducing apoptic cells at earlier stage. Conclusions As a non-competitive inhibitor of EAAs-R, MgSO4, hasprotective effect during cerebral ischemia reperfusion, but the protecive effect has no relation with apopticcontrol.