小红细胞对拟合曲线法与移动浮标法计数血小板结果的影响探讨

The Influence of m-Microcyte on Platelet-counting of Hematology Analyzers of Ftting Curve and Floating Threshold

[目的]探讨不同平均红细胞体积(MCV)参数的小红细胞对拟合曲线法与移动浮标法计数血小板结果的影响。[方法]从日常检测的患者标本中,选取MCV<60fL的标本42例,MCV在60～70fL的标本63例,MCV在70～80fL的标本89例,分别在采用拟合曲线法的贝克曼LH750和采用移动浮标法的希斯美康XS-800i血细胞分析仪上进行血小板(PLT)计数,并对每份标本行手工显微镜镜检计数二次取均值统计。仪器与手工组间的比较采用配对t检验。[结果]对MCV在70～80fL的标本,拟合曲线法和移动浮标法的PLT结果与手工镜检法PLT结果差异无统计学意义(P>0.05);当MCV在60～70 fL之间时,拟合曲线法与镜检法之间PLT计数结果差异无统计学意义(P>0.05),但移动浮标法PLT计数结果明显高于镜检法(P<0.01);当MCV<60fL时,拟合曲线法与镜检法之间PLT计数结果差异无统计学意义(P>0.05),但移动浮标法PLT计数结果明显高于镜检法(P<0.01)。[结论]血细胞分析仪在进行PLT计数时应关注MCV参数。对使用移动浮标法的仪器,在MCV≤70fL时应通过手工镜检复查血小板计数结果;而使用拟合曲线法的仪器,则能有效避免小红细胞对血小板计数结果的影响。

[Objective] To investigate the effect of microcyte of different MCV on the PLT counting using hematology analyzer according to fitting curve and floating threshold in platelet decreased sample.[Methods] All platelet tested patients＇ samples,which include 46 sample with MCV（mean corpuscular volume） 60fL,77 sample with MCV 60～70fL and 78 sample with MCV 70～80fL,are analyzed separately by Beckman Coulter LH750 and Sysmex XS-800i.The platelet is counted twice with manual method and then take mean statistical.Paired t test is used to compare the measurement data among groups.[Results] Platelet counts in fitting curve,floating threshold and manual method groups in which the MCV is between 70 and 80 fL,are of no statistical significance;the PLT results in hand work group are similar with those in fitting curve group when MCV is between 60 and 70 fL,but which are significantly lower than in floating threshold group;if the MCV was less than 60 fL,the PLT results in hand work group are similar with those in fitting curve group,but which are significantly lower than in floating threshold group.[Conclusions] MCV influence should be concerned while counting PLT with Hematology Analyzers.We should recount the platelet with manual method when MCV ≤ 70fL by floating threshold Hematology Analyzer.Fitting curve Hematology Analyzer could avoid the influence of microcyte on platelet-counting.