微生物絮凝剂絮凝特性研究

Microbial Flocculants Flocculation Properties Research

目的研究微生物絮凝剂絮凝菌株S-3的絮凝条件对絮凝活性的影响,确定微生物絮凝剂的最佳絮凝条件.方法改变高岭土悬浊液的pH值、温度条件和生物絮凝剂的加入量来测定高岭土悬浊液的吸光度值.结果絮凝活性物质主要存在于发酵液中,微生物絮凝剂在pH值为8条件下絮凝活性最高.絮凝剂对温度的变化较敏感,对温度表现出热不稳定性,随着温度的升高絮凝活性降为0,加入6 mL Ca2+离子后絮凝活性提高为92.4%.絮凝活性在一定范围内随着絮凝剂添加量的增加而提高,加入1～2 mL的微生物絮凝剂时絮凝率最高为93.1%.结论由实验室制得的微生物絮凝剂的活性物质主要存在于离心上清液中,高岭土悬浊液絮凝体系的环境对微生物絮凝剂的絮凝效果有影响作用,微生物絮凝剂的主要成分是蛋白质.

The study aims to research the microbial flocculants flocculation strains S-3′s influence on the flocculation activity under different flocculation conditions and identify the best flocculation conditions of the microbial flocculants.The major method is respectively changing the pH value,temperature condition and volume of addition of the kaoline soliquoid and recording their absorbance values by using spectrometers.The experiment results show that flocculation active substances mainly exist in fermented liquid,microbial flocculants keep the highest activity when the pH value of the kaoline soliquoid is 8.Meanwhile,microbial flocculants are sensitive to the changing of the temperature and present great instability.With the rise of the temperature,flocculation activity of the microbial flocculants reduced to 0 and flocculation activity increase to 92.4% when 6 mL Ca2＋ is added into the kaoline soliquoid.Flocculation activity increases with the improvement of the volume of flocculants in a certain range and adding 1~2 mL microbial flocculants contributes to the result that the highest rate of flocculation reaches to 93.1%.We can make the conclusion as follows.Active materials of the microbial flocculants mainly exist in the centrifugal supernatants.The flocculants system environment of the kaoline soliquoid makes a significant role in the flocculation effect of the microbial flocculants and microbial flocculants consists mainly of protein.