洛沙坦对血管平滑肌细胞增殖的干预作用及C-myc基因表达的影响

Influence of Losartan on Proliferation of Cultured Vascular Smooth Muscle Cells with Correlation to Gene Expression of C myc

目的】探讨血管紧张素Ⅱ受体拮抗剂洛沙坦(losartan)在高血压病血管重塑过程中的作用及作用机制。【方法】①应用ACAS570共聚激光扫描显微系统,通过荧光免疫组化方法,对洛沙坦作用下体外培养的大鼠血管平滑肌细胞中的核细胞增殖抗原(PCNA)及与增殖相关的Cmyc基因表达产物进行定量检测。②测定洛沙坦作用下的体外培养的大鼠血管平滑肌细胞的增殖活性(MTT)值。【结果】洛沙坦作用下血管平滑肌细胞的增殖活性(MTT)降低;实验组和对照组分别为02045±00013,02239±00010(P<005)。PCNA及Cmyc的表达都明显降低;实验组和对照组PCNA分别为1338.56±232.56,154270±20004(P<001)。Cmyc分别为152803±12514,174035±20591(P<001)。【结论】洛沙坦通过影响血管平滑肌细胞的增殖活性而参与血管壁结构的改变。

Objective To explore the effect of losatan (an angiotensin Ⅱ receptor blocking agent) on the vascular remoulding in hypertension and its mechanism. Method ①Cofocusing laser scanning microscope system (Model ACAS570) was used, and by the method of fluorescent immunochemistry, the proliferative cell nuclear antigen(PCNA) and the proliferation related expression product of C myc gene in cultured rat vascular smooth muscle cells treated with losatan were quantitatively assayed. ②The proliferative activity (MTT value) of the vascular smooth muscle cells treated with losartan was analyzed. Result Under the influence of losatan, the vascular smooth muscle cells′ proliferative activity (MTT) decreased, the values in the groups of experiment and control were 0 2 045±0 0 010 and 0 2 239±0 0 010 (P<0 05), respectively. The cellular PCNA and the expression of C myc also decreased significantly, PCNA in the groups of experiment and control were 1 338 56±232 56 and 1 542 71±200 04 (P<0 01); and C myc expression in the two groups were 1 528 03±125 14 and 1 740 35±205 91(P<0 01),respectively. Conclusion Losartan takes part in the changes of the vascular wall structure through effecting on the porliferative activity of vascular smooth muscl cells.