铁皮石斛菌根真菌对铁皮石斛组培苗的接种效应

Inoculation Effects of Dendrobium officinale Mycorrhizal Fungi on Their Plantlets

[目的]对铁皮石斛(Dendrobium officinale)菌根真菌对铁皮石斛组培苗的接种效应进行研究。[方法]以从野生铁皮石斛根系中分离得到的Tj1、Tj2、Tj3真菌为材料,将其接种到铁皮石斛组培苗的根系上进行接种试验。[结果]在组培条件下,Tj1菌在前期没有表现出促生作用,Tj2菌表现出较强的促生作用,Tj3菌则对根具有促生作用,而在后期,Tj1菌则表现出了较强的促生作用,Tj2菌的接种效果最好,促生作用最强,Tj3菌则出现烂根烂苗现象;在室外条件下,Tj2菌接种使组培苗叶数增加,侧芽数增加,侧根生长明显,株高增加显著,叶片大而深绿,根部有明显的膨大现象。[结论]2种接种方式均说明Tj2菌对铁皮石斛的地上植株和地下根系生长,株数增多,株高增加,新芽和新根萌发均具有较强的促进作用,证明Tj2菌与铁皮石斛有效地建立了共生关系。因此Tj2菌对石斛组培苗的成功栽培具有实际应用价值。

[Objective] This study aimed to explore the inoculation effects of Dendrobium offwinale mycorrhizal fungi on their plantlets. [ Method] Endophytic strains Tjl, Tj2 and Tj3were obtained by isolation and purification from mycorrhiza of wild Dendrobium offtcinale and inoculated into the root system of Dendrobium officinale for inoculation test. [ Result ] Under tissue-culture conditions, at early stage, Tjl strain hadn＇ t shown promotion effect on Dendrobium officinale, Tj2 strain had shown relatively strong promotion effects, and Tj3 strain had promoted the growth of roots; at late stage, Tjl strain had shown relatively strong promotion effects, Tj2 strain had shown the best inoculation effects and the strongest promotion effects, while Tj3 strain had caused root and seedling rot problems of the plantlets ; under outdoor conditions, after in- oculation with Tj2 strain, the number of leaves and lateral buds were increased, the growth of lateral root and the increase of plant height were significant, the leaves of Dendrobium officinale plantlets were large and dark green and an obvious root enlargement phenomenon was observed. [ Conclusion ] The two inoculation methods both indicated that Tj2 strain had relatively strong promotion effects on the growth of Dendrobium of- ficinale roots and shoots, the increase of plant number and plant height, and the germination of new shoots and roots, which proved the effective establishment of symbiotic relationship between Tj2 strain and Dendrobium officinale. Therefore, T2 strain had practical application values on the successful cultivation of Dendrobium officinale plantlets.