鸡大肠杆菌重组1型菌毛疫苗的免疫原性

Immunogenicity of Vaccine against Recombinant Type 1 Pilus of Avian Escherichia coli in Chickens

[目的]制备鸡大肠杆菌重组1型菌毛疫苗,并探索其免疫原性。[方法]从含1型菌毛的基因重组菌株CZYR10与野生型鸡大肠杆菌分离株YR(018)中提取1型菌毛,制备菌毛油乳剂疫苗。通过对鸡群的攻毒试验,探讨该疫苗的免疫原性。[结果]重组1型菌毛蛋白具有免疫保护作用。重组1型菌毛疫苗的保护力比分离的鸡大肠杆菌1型菌毛疫苗保护率低,但差异不显著(P>0.05)。[结论]该研究可为鸡大肠杆菌病的免疫防治提供依据。

[ Objective ] To prepare vaccine against recombinant type 1 pilus protein of avian Escherichia coli and to detect its immunogenicity in chickens. [ Method] The type 1 pilus was respectively isolated from the recombinant bacteria CZYR10 strain and wild avian Escheriehia coli YR（018） strain and used to prepare oil- emulsified vaccine. The immunogenicity of the developed vaccine was detected in chickens using challenge test. [ Result ] The recombinant type 1 pilus protein had immunoprotective efficacy. The recombinant type 1 pilus protein had weaker protective efficacy then the isolated type 1 pilus protein, but the difference was not significant. [ Conclusion ] The study provides a reference for immunization and control of chicken colibacillosis.