黑米花青素提取与纯化工艺研究

Study on Extraction and Purification Technology of Anthocyanin from Black Rice

[目的]研究黑米花青素的提取及纯化工艺。[方法]以黑米为原料,采用水提法提取黑米花青素,通过单因素试验和正交试验,确定花青素的最佳提取工艺;并采用D101大孔吸附树脂对花青素进行纯化。[结果]花青素的最佳提取工艺为料液比1∶8,提取温度50℃,pH 3.2,提取时间120 min;用体积分数为95%的乙醇洗脱,花青素的纯度最高,达95.48%。[结论]该研究为黑米的深加工和花青素的规模化生产提供了依据。

[ Objective ] The aim was to study the extraction and purification technology of anthocyanin from black rice. [ Method ] Anthocyanin was obtained from black rice by water extraction. The optimum extraction technology of anthocyanin was determined by single factor experiment and orthogonal experiment. And then the anthocyanin was purified by D101 macroporous adsorption resin. [ Result] The results showed that the optimum extraction conditions were as follows ： solid-liquid ratio 1 ： 8, extraction temperature 50℃, pH value 3.2, extraction time 120 min . The highest purity reached 95.48% when anthocyanin eluted by 95% ethanol. [ Conclusion] The study provided a basis for deep processing of black rice and scale production of anthocyanin.