重组人乳铁蛋白对原代肝·肾细胞的毒性研究

Cytotoxicity research of Recombinant Human Lactoferrin on Primary Hepatocyte and Nephrocyte Cell

[目的]通过模拟肝、肾毒性的体外细胞试验,研究重组人乳铁蛋白是否对小鼠原代肝肾细胞具有毒性作用,为其进一步的安全性评价提供依据。[方法]采用模拟体外消化试验所得的重组人乳铁蛋白消化产物及重组人乳铁蛋白为受试物,牛乳铁蛋白为对照,以小鼠原代肝、肾细胞为平台,用MTT法检测受试物对细胞活性的半数抑制浓度(IC50),研究细胞毒性剂量-反应关系。[结果]在设定的试验条件下,未发现重组人乳铁蛋白对肝、肾细胞存在靶毒性,结果能与已进行的亚慢性毒性试验相互印证。[结论]为重组人乳铁蛋白的进一步安全性评价及其他转基因食品的安全性评价方法提供了参考。

[ Objective] The aim was to study on whether recombinant Human Lactoferrin has toxic effect on Primary Hepatocyte and Nephrocyte Cell of rat to provide reference for further safety evaluation. [ Method ] Recombinant Human Lactoferrin and its digested products were taken as tested compound, cow Lactoferrin was used for contrast. Primary Hepatocyte and Nephrocyte Cell of rat werecultured and 1C50 values were tested by MTF,and cytotoxic dose-response relationship wastested. [ Result] Target toxicity was not found from recombinant Human Lactoferrin on hepatocytesand nephrocytes, in accordance with sub-chronic toxicity test. [ Conclusion ] This study is of reference value for further safety evaluation of recombinant Human Lactoferrin and safety of evaluation method of GM food.