高脂饮食对大鼠胸主动脉瘤形成过程中细胞黏附分子表达的影响

Effect of high-fat diet on cell adhesion molecules expression in the thoracic aortic aneurysm

目的探讨高脂饮食对胸主动脉瘤形成过程中细胞黏附分子包括血管细胞黏附分子-1(VCAM-1)和细胞间黏附分子-1(ICAM-1)表达的影响。方法将30只成年雄性Wistar大鼠随机分为3组,即对照组、手术组和高脂饮食组,采用氯化钙(CaCl2)诱导法制备胸主动脉瘤模型,于术后4周取外敷CaCl2段胸主动脉。采用地衣红染色和HE染色观察胸主动脉形态学改变;免疫组织化学技术检测CD45、VCAM-1和ICAM-1的表达。结果高脂饮食组动脉管径较手术组扩张明显,动脉管壁破坏更广泛,伴有更多的炎性细胞浸润;VCAM-1、ICAM-1较手术组表达升高。结论高脂饮食可促进胸主动脉瘤管壁VCAM-1和ICAM-1的表达。

Objective To investigate the effect of high-fat diet on expression of cell adhesion molecules （ VCAM- 1, ICAM-1） during thoracic aortic aneurysm formation. Methods Thirty adult male Wistar rats were randomly divided into three groups： sham group, surgery group and high fat group. The thoracic aortic aneurysm model was induced by CaC12. The thoracic aortic segments were collected 4 weeks after surgery. Orcein staining and HE staining were used to observe the morphological changes in thoracic aorta. Immunohistochemistry was performed to detect the expression of CD45,VCAM-I and ICAM-1. Results Compared with surgery group, the high fat diet group had more obvious dilation of arterial diameter, more extensive damage to the arterial wall which was associated with more serious inflammatory cell infiltration and the increased expression of VCAM-1, and ICAM-1. Conclusion High-fat diet promotes the VCAM-1 and ICAM-1 expression in thoracic aortic aneurysm wall, which may play an important role in the formation of thoracic aortic aneurysm.