兔实验性非侵袭性真菌性上颌窦炎模型的制备

A new method to create experimental model of noninvasive fungal sinusitis in rabbits

目的探讨实验性非侵袭性真菌性上颌窦炎动物模型的制备方法。方法 25只新西兰大白兔(共50侧鼻窦)分为7组:空白对照组(6侧)、假手术对照组(6侧)、黏膜划伤组(6侧)、窦口阻塞组(6侧)、仅注入烟曲霉菌组(8侧)、注入烟曲霉菌并给予黏膜划伤组(9侧)、注入烟曲霉菌加窦口阻塞组(9侧)。以上颌窦自然开口是否予以封闭以及鼻窦黏膜是否划伤作为分组标准。观察以上组别在接种烟曲霉菌后成为真菌性鼻窦炎的可能性。于接种后14d及28d,根据上颌窦内分泌物真菌培养、分泌物涂片及病理检查结果判定各组制模成功率。结果注入烟曲霉菌加窦口阻塞组上颌窦分泌物烟曲霉菌培养阳性率14d为88.9%(8/9侧),28d为77.8%(7/9侧)。而注入烟曲霉菌并给予黏膜损伤组上颌窦分泌物烟曲霉菌培养阳性率14d为44.4%(4/9侧),28d为22.2%(2/9侧)。结论窦口阻塞加注入烟曲霉菌的方法可成功制造实验性非侵袭性真菌性上颌窦炎模型。

Objective To investigate a new method for creating experimental model of noninvasive fungal sinusitis in rabbits. Methods Twenty-five New Zealand white rabbits （50 sides）were divided into seven groups., control group （n = 6 sides）, sham-operation group （n= 6 sides）, mucosal-scratched group （n= 6 sides） ,ostia-blocked group（n= 6 sides） , Aspergillus furnigatus inoculated group（n ： 8 sides）, mucosal-scratched plus Aspergillus fumigatus inoculated group（n = 9 sides）, and ostia-blocked plus Aspergillus fumigatus inoculated group（n：9 sides）. The experiment was divided according to whether the maxillary sinus mucosa was wounded or not and whether the natural ostia were blocked or not. On days 14 and 28 after Aspergillus furnigatus inoculation, histopathologic and mycological examinations of both maxillary cavities and mucous membrane were performed to confirm the establishment of the model. Results Aspergillus fumigatus plus ostial obstruction group had a positive rate of 88.9% （8/9 sides） on day 14 and 77. 8% （7/9 sides） on day 28 in fungal culture. Aspergillus furnigatus plus mucosa wound yielded a positive rate of 44. 4% （4/9 sides） on day 14 and 22. 2% （2/9 sides） on day 28. Conclusion Aspergillus fumigatus inoculation combined with an blocked ostium can be used to establish experimental model of noninvasive maxillary fungal sinusitis.