摘要
目的比较脂肪源间充质干细胞(AMSCs)和骨髓来源间充质干细胞(BMSCs)的免疫调节能力的差异。方法用流式细胞仪分析AMSCs和BMSCs的表型。通过MSCs和淋巴细胞共培养体系,检测MSCs对T细胞增殖、周期、活化、凋亡以及Th细胞分化的影响。结果表型分析结果显示AMSCs和BMSCs的表型基本一致。AMSCs和BMSCs都能抑制T细胞的增殖和早期活化,并在调节辅助性T细胞亚群的分化上作用类似。但在MSC对活化后T细胞的凋亡影响方面,BMSCs能够抑制活化T细胞的凋亡,而AMSC没有这种作用。结论 AMSCs和BMSCs对T淋巴细胞的影响基本类似,其对活化T细胞凋亡影响的差异还有待进一步的机制研究。
Objective To compare MSCs＇ immuno-modulatory property between bone-marrow derived and adipose derived MSCs. Methods Phenotype analysis of both MSCs populations was detected by flow-cytometry. To analyse immuno-modulatory effect of MSCs, proliferation, cell cycle, activation, apoptosis and Thl/Th2 balance were ana- lyzed after co-culturing lymphocytes and MSCs. Results Phenotype analysis showed that AMSCs had the similar ex- pression of markers with BMSCs. Both AMSCs and BMSCs could inhibit T cells proliferation and activation. Also MSCs＇ effect on Th cell differentiation into Thl or Th2 was similar between AMSCs and BMSCs. Nevertheless, BMSCs could suppress activated T cell apoptosis, while AMSCs could not. Conclusions AMSCs and BMSCs showed similar immuno-modulatroy effect on T lymphocytes, however the difference effect on activated T cell apop- tosis of the two cell populations is still unclear and the mechanism needs to explore.
出处
《基础医学与临床》
CSCD
北大核心
2012年第2期128-132,共5页
Basic and Clinical Medicine
基金
国家自然科学基金青年基金(30800429)
"长江学者和创新团队发展计划"项目(IRT0909)
