期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

四环素基因调控系统的研究进展 被引量：6

New strategies in tetracycline-regulatable system
下载PDF
导出
摘要 四环素基因调控系统(Tet系统)是迄今为止基因治疗研究中使用最广泛的系统,该系统通过改变培养基、体内四环素或其衍生物(如强力霉素)的浓度,诱导或抑制目标基因的表达。 The tetracycline regulatory system （Tet system） adjusting the concentration of tetracycline or its derivatives sion of target gene will be switched on or off. is by far the most frequently used in gene therapy. By （such as doxycyline） in medium or in vivo,the expres-
作者 宫秀群 马敏敏 徐格林
机构地区 南京大学医学院临床学院 南京军区南京总医院神经内科
出处 《基础医学与临床》 CSCD 北大核心 2012年第2期233-236,共4页 Basic and Clinical Medicine
基金 国家自然科学基金(30870848) 南京军区南京总医院医学科研课题(Q2008022)
关键词 基因调控 四环素基因调控系统 基因治疗 gene regulation tet system gene therapy
分类号 R346 [医药卫生—基础医学]
  • 相关文献

参考文献15

  • 1Xie J,Nair A,Hermiston T.A comparative study examining thecytotoxicity of inducible gene expression system ligands in dif -ferent cell types[J].Toxicol in Vitro,2008,22:261-266.
  • 2Hackl H,Rommer A,Konrad TA,et al.Tetracycline regula-tor expression alters the transcriptional program of mammali-an cells[J].PLoS One,2010,5:e13013.
  • 3Doring A,Pfeiffer F,Meier M,et al.TET inducible expres-sion of the alpha4beta7-integrin ligand MAdCAM-1 on theblood-brain barrier does not influence the immunopathogene-sis of experimental autoimmune encephalomyelitis[J].Eur JImmunol,2011,41:813- 821.
  • 4Yu J,Muller H,Hehn S,et al.Construction and applicationof an inducible system for homogenous expression levels inbulk cell lines[J].PLoS One,2009,4:e6445.
  • 5Alexeyev M F,Fayzulin R,Shokolenko I N,et al.A retro-lentiviral system for doxycycline-inducible gene expressionand gene knockdown in cells with limited proliferative capac-ity[J].Mol Biol Rep,2010,37:1987-1991.
  • 6Konopka W,Duniec K,Klejman A,et al.Tet system in thebrain:transgenic rats and lentiviral vectors approach [J].Genesis,2009,47:274-280.
  • 7Sheng Y,Lin CC,Yue J,et al.Generation and characteriza-tion of a Tet-On(rtTA-M2)transgenic rat[J].BMC DevBiol,2010,10:17.
  • 8Yang X,Mertens B,Lehtonen E,et al.Reversible neuro-chemical changes mediated by delayed intrastriatal glial cellline- derived neurotrophic factor gene delivery in a partialParkinson's disease rat model[J].J Gene Med,2009,11:899-912.
  • 9Groner AC,Meylan S,Ciuffi A,et al.KRAB-zinc finger pro-teins and KAP1 can mediate long-range transcriptional re-pression through heterochromatin spreading[J].PLoS Gen-et,2010,6:e1000869.
  • 10Eger K,Hermes M,Uhlemann K,et al.4-Epidoxycycline:an alternative to doxycycline to control gene expression inconditional mouse models[J].Biochem Biophys Res Com-mun,2004,323:979-986.

同被引文献49

  • 1王恒安,秦磊,庞志轩,赵国屏.吸水链霉菌TetR基因的克隆与表达[J].吉林农业大学学报,2004,26(3):280-283. 被引量：1
  • 2徐宗全,陈孝平,张万广,李常海,李高鹏.Tet-on基因表达系统调控HIF-1α表达对肝癌细胞凋亡的影响[J].中华普通外科杂志,2006,21(11):769-772. 被引量：3
  • 3陈维刚,王强,牛李丽,赵波,余星明,邓家波.赤麻鸭类志贺邻单胞菌感染的诊治[J].四川畜牧兽医,2006,33(12):47-47. 被引量：5
  • 4Reboredo M, Kramer MG, Smerdou C, et al. Transcriptomie effects of Tet-on and mifepristone-inducible systems in mouse liver [J]. Human Gene Ther. 2008.19 ( 11 ) : 1233-1247.
  • 5Han Y, Chang QA, Virag T, et al. Lack of humoral inunune response to the tetracycline (Tet) activator in rats injected intracranially with Tet-off rAAV vectors[J]. Gene Ther,2010,17(5) :616-625.
  • 6Huang Z, Moya C, Jayaraman A, et al. Using the Tet-on system to develop a procedure for extracting transcription Lactor activalion dynamics [ J ].Mol Biosyst, 2010,6 ( 10 ) : 1883-1889.
  • 7Stary E, Gaupp R, Leehner S, et al. New architectures for Tel-on and Tet-off regulation in Staphylococcus aureus [J ]. Appl Environ Microbiol, 2010,76 ( 3 ) : 680-687.
  • 8Wen Y, Lan J, Huang H, et al. Application of eGFP to label human periodontal ligament stem cells in periodontal tissue engineering[J]. Arch Oral Biol,2012,57(9) : 1241-1250.
  • 9Whyte J, Laughlin MH. Placentation in the pig visualized by eGFP fluorescence in eNOS over-expressing cloned transgenic swine[J]. Mol Reprod Dev,2010,77(7):565.
  • 10Gossen M, Freundlieb S, Bender G, et al. Transcriptional activation by tetracycline in mammalian eells[J]. Science, 1995,268(5218) : 1766-1769.

引证文献6

二级引证文献9

基础医学与临床
2012年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部