宽体金线蛭抗凝物质的提取与纯化

Extraction and Purification of the Element of Anticoagulant from Whitmania pigra Whitman

采用丙酮三氯乙酸法、凝胶过滤层析、阴离子交换层析、Sep-pak C18柱宽体金线蛭中抗凝物质进行分离与纯化,并且进行SDS-PAGE凝胶电泳。结果表明:SDS-PAGE电泳为单一条带,并且提取的物质具有抗凝活性,测序结果表明得到一种新的抗凝物质。

By dint of acetone and trichloracetic acid,Sephadex G-50,DEAE-cellulose column and Sep-pak to isolate and purify and using SDS-PAGE polyacrylamide gel electrophoresis,it was found that SDS-PAGE polyacrylamide gel electrophoresis had only one band and the purified element had anti-freezing,and the element was a new protein.