二氧化碳气调对3种中药材储藏期害虫SOD酶活性的影响

Effect of Controlled Atmosphere of Carbon Dioxide(CO_2) on Superoxide Dismutase(SOD) Activity of Three Storage Pests in Chinese Medicinal Materials

[目的]研究二氧化碳气调胁迫对昆虫机体SOD酶活性的影响,以分析气调胁迫对昆虫作用的生物学机制。[方法]采用氯化硝基四氮唑蓝光还原法,研究药材甲、烟草甲、和咖啡豆象3种昆虫SOD酶的活性,分析该酶在CO2气调胁迫下的应激反应。[结果]高浓度二氧化碳气调胁迫处理3、6 h时,药材甲、烟草甲和咖啡豆象成虫SOD酶活力均有一定程度的持续升高,其中处理6 h,其活力升高显著,分别由对照2.011±0.954、2.664±0.218、1.458±0.718 U/(头.30 m in)上升到3.135±0.105、3.050±0.673、2.975±0.229 U/(头.30 m in)。[结论]在亚致死事件范围内,高浓度二氧化碳气调胁迫对中药材储藏期害虫SOD酶活力有一定激活作用。研究结果可丰富气调杀虫机理及昆虫抗气性分析的理论体系。

[Objective]The paper was to study the effect of controlled atmosphere stress of carbon dioxide（CO2） on Superoxide dismutase（SOD） activity of insect,so as to analyze the biological mechanism of the action of controlled atmosphere stress on insect.[Method]Using nitrotetrazolium blue chloride（NBT） light reduction method,SOD activity of drugstore beetle（Stegobium paniceum）,cigarette beetle（Lasioderma serricorne） and coffee bean beetle（Araecerus fasciculatus） was studied,and the stress response of the enzyme under controlled atmosphere stress of CO2 was analyzed.[Result]SOD activity of drugstore beetle,cigarette beetle and coffee bean beetle exposed to controlled atmosphere stress of high concentrations of CO2 for 3 and 6 h had certain degree of increase,and the activity significantly increased from 2.011±0.954,2.664±0.218 and 1.458±0.718 to 3.135±0.105,3.050±0.673 and 2.975±0.229 U/（head·30 min）after exposure for 6 h.[Conclusion]Controlled atmosphere stress of high concentrations of CO2 had certain activation effect on SOD activity of storage pest in Chinese medicinal material within the context of sub-lethal events.The results could enrich the insecticidal mechanism of controlled atmosphere and theoretical system of analysis on insect resistance to atmosphere.