响应曲面法优化溶藻细菌发酵条件的研究

Optimization of Fermentation Condition of Algicidal Bacteria using Response Surface Methodology

[目的]为探讨溶藻细菌对水华蓝藻的去除效应,为溶藻细菌规模化发酵提供理论基础和技术依据,对一株溶藻细菌R2的发酵条件进行了优化。[方法]采用响应曲面法对该试验室分离的一株溶藻细菌R2的发酵条件进行了优化。首先通过全因子试验分析了培养温度、初始pH、摇床转速、装液量、接种量对溶藻细菌生长的影响;用最陡爬坡实验逼近最大响应区域,利用中心组合设计及响应面分析确定主要影响因子的最佳水平。[结果]确定的溶藻细菌R2最优化发酵条件为:温度31.8℃、pH 7.21、摇床转速180 r/m in、装液量60%、接种量10%。在优化的发酵条件下,培养24 h溶藻细菌OD600增加值可达2.310。在优化条件下发酵的溶藻细菌进行溶藻效果检测,72 h后铜绿微囊藻叶绿素a去除率达78%。[结论]采用响应曲面法能够有效的优化溶藻细菌的发酵条件,在最优化发酵条件下溶藻细菌生长情况良好,其溶藻效应也可达到一定水平。

[Objective]To investigate cyanobacteria bloom removal effect of the algicidal bacteria and provide the theoretical and technical basis for the algicidal bacteria,the fermentation condition of the algicidal bacteria R2 were optimized.[Method]Response surface methodology was employed to optimize the fermentation conditions of algicidal bacteria R2 which separated by the lab.A full factorial design was used to investigate the effect of temperature,initial pH,wave speed,inoculum and medium volume on the growth of algicidal bacteria.[Result] The temperature and initial pH had significant effect on protease production,both of them had positive effect.The optimized fermentation conditions of the algicidal bacteria R2 were temperature 31.8;pH 7.21;shake speed 180 r/min;mediun volume 60%;inoculum 10%.The maximum increase of OD600 of algicidal bacteria was 2.310 under the optimized conditions after 24 h.Under the optimum conditions for fermentation,the removal rate of Chl-a was 78%.[Conclusion]Using respone surface methodology can effectively optimize the fermrntation conditions of the algicidal bacteria.On the optimized fermentation conditions,the growth condition and the lytic effect of the algicidal bacteria can reach a certain level.