用于假体固定的骨基质明胶复合骨水泥超微结构与成骨活性分析

Ultrastructure Characteristic and Osteogenesic Bioassay on BMG/PMMA Compound Materials for Joint Prosthesis Fixation

目的通过观察新复合材料超微结构与成骨活性,探讨其用于人工关节假体固定的可能性。方法将自制的兔骨基质明胶(bone matrix gelatin,BMG)与聚甲基丙烯酸甲酯骨水泥(polymethyl methacrylate,PMMA)等质量比复合,制成复合材料,采用扫描电镜观察其结构特点,将复合材料植入兔臀部肌肉中观察其成骨性能。结果在复合材料表面及内部,见100～300μm的不规则孔隙,相互贯通;直径为100～400μm的BMG颗粒分布不均匀。术后4周,光镜下可见肌组织内明显成骨现象。结论 BMG/PMMA复合材料的多微孔结构及其诱导成骨效应,为其用于假体固定提供了可能性。

Objective To explore the possibility of joint prosthesis fixation with new compound materials through observing its ultrastructure and osteogenesis. Methods The materials was composed of self-made rabbit bone matrix gelatin（BMG） and polymethyl methacrylate（PMMA） bone cement mixed according to the mass ratio 1∶1.The structural features and the in vivo osteogenesis activity of the compound after implantation in the buttocks muscle in rabbits were observed by the scanning electron microscope. Results On the surface and inside of the compound materials,a lot of irregular interconnected pores with 100-300 μm diameters,and many BMG particles distributing unevenly with 100-400 μm diameters were observed.Obvious osteoinduction in the rabbit muscle was observed under the light microscope at the fourth week after the operation. Conclusion The multiple micropore structure and osteoinduction effect of the BMG/PMMA compound materials make it possible for the application for artificial prosthesis fixation.