CTLA-4基因单倍型与吸毒合并HIV感染的相关性研究

Association between Cytotoxic T Lymphocyte-associated Antigen 4 Gene Haplotype and Drug Abuse with HIV Infection

目的研究细胞毒T淋巴细胞相关抗原4(cytotoxic T lymphocyte associated antigen-4,CTLA-4)基因外显子1的49位点和启动子-318位点单倍型与吸毒合并HIV感染的相关性。方法采用序列特异性引物聚合酶链反应(polymeriase chain reaction with sequence-specific primers,PCR-SSP)方法,检测24例单纯吸毒者、41例吸毒合并HIV感染患者以及204例正常对照者CTLA-4基因外显子1的49位点启动子-318位点的单倍型。结果吸毒者中CTLA-4A+49G和C-318T单倍型与正常对照组间差异无统计学意义(P>0.05)。吸毒合并HIV感染组中CTLA-4单倍型1.1(C-318-A+49/C-318-A+49)显著低于对照组(2.4%vs 13.2%,P<0.05);CTLA-4单倍型3.3(T-318-A+49/T-318-A+49)明显高于对照组(12.2%vs 2.9%,P<0.01)。与吸毒组比较,吸毒合并HIV感染组中CTLA-4单倍型1.1(C-318-A+49/C-318-A+49)显著下降(2.4%vs 12.5%,P<0.05)。结论吸毒合并HIV感染与CTLA-4基因动态密切相关,单倍型1.1与吸毒合并HIV感染呈负相关,单倍型3.3与吸毒合并HIV感染呈正相关。

Objective To investigate the association between the haplotype of cytotoxic T lymphocyte-associated antigen 4（CTLA-4） and drug abuse with HIV infection. Methods The A＋49G transition polymorphism at position 49（exon 1） and C-318T transition polymorphism at position 318 in the promoter of the CTLA-4 gene were determined by polymerase chain reaction with sequence specific primers（PCR-SSP） method in 24 drug abusers,41 drug abusers with HIV infection and 204 healthy controls. Results Compared with the control group,no significant difference in the distribution of haplotype was observed in A＋49G and C-318T gene polymorphisms in drug abusers（P0.05）,the frequency of haplotype 1.1（C-318-A＋49/C-318-A＋49） in drug abusers with HIV infection was significantly lower（2.4% vs 13.2%,P0.05）,and the frequency of haplotype 3.3（T-318-A＋49/T-318-A＋49） was significantly higher（12.2% vs 2.9%,P0.01）.Compared with drug abusers,the frequency of haplotype 1.1（C-318-A＋49/C-318-A＋49） was significantly lower in drug abusers with HIV infection（2.4% vs 12.5%,P0.05）. Conclusion There exists close relation between CTLA-4 alleles and drug abuse with HIV infection.Haplotype 1.1 is negatively and haplotype 3.3 is positively correlated with drug abuse with HIV infection.