摘要
研究了固相萃取(SPE)GCMS法同时测定葡萄酒中顺反式白藜芦醇的分析方法.用SPEC18小柱进行葡萄酒样品的前处理,以2三甲基甲硅烷基三氟乙酰胺(BSTFA)试剂衍生化分离出的白藜芦醇,用GCMS法对顺、反式白藜芦醇进行定性和定量分析.对顺、反式白藜芦醇方法的检出限分别为372μg·L-1和600μg·L-1,平均回收率分别为939%和988%,RSD分别为74%和81%.分析了31个国产葡萄酒,11个进口葡萄酒.该法简便、快速、损失小,样品用量少,适合大批量样品的测定.
A novel method for the determination of resveratrol in wine was developed Solid\|phase\|extraction (SPE) was carried out on a SPE C\-\{18\} column N,O\|Bis(trimethylsilyl) trifluoroacetamide (BSTFA)was used for sample derivatization Sample aliquots were analyzed qualitatively and quantitatively with GC-MS The detection limits, recovery ranges, and precision were 3 72 μg·L\+\{-1\}, 86% to 103%, and 4 5% for\% cis\%\|resveratrol respectively, 6 00 μg·L\+\{-1\}, 91% to 110% and 5 2% for \%trans\%\|resveratrol respectively Thirty\|one homemade wines and 11 import wines were analysed This method was inexpensive, quick, specific, selective and needs minimal amounts of sample
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2000年第1期63-67,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
关键词
固相萃取
葡萄酒
白黎芦醇
SPE-GC-MS法
顺反式
solid\|phase\|extraction(SPE)
gas chromatography\|mass spectrometry(GC\|MS)
wine
resveratrol
