自体血清皮肤试验阳性慢性荨麻疹与HLA—DRB1基因的相关性研究

Relationship between HLA-DRB1 alleles and chronic urticaria with positive autologous serum skin test

目的 探讨广西地区白体血清皮肤试验阳性慢性荨麻疹与HLA-DRB1等位基因遗传易感性的关系.方法 对144例广西地区慢性荨麻疹患者进行自体血清皮肤试验,按试验结果分为阳性组62例,阴性组82例.采用聚合酶链反应-序列特异性引物方法,对患者组和199例正常人对照组进行HLA-DRB1等位基因的分型,并分析DRB1基因在3个组中的分布.使用SPSS13.0统计软件分析.结果 DRB1＊01、＊1401、＊16等位基因频率在阳性组、阴性组和正常人对照组间比较,差异均有统计学意义（x2=10.92,Pc=0.03;x2=35.34,Pc＜0.01;x2=12.69,Pc=0.03）.进一步在各组间进行两两比较,仅DRB1＊1401等位基因频率在自体血清皮肤试验阳性组与对照组间（RR=17.09,Pc＜0.01）及自体血清皮肤试验阳性组与阴性组间（RR=7.20,P＜0.01）,差异均有统计学意义.结论 DRB1＊1401等位基因可能是广西地区自体血清皮肤试验阳性慢性荨麻疹的易感基因或与其连锁.

Objective To investigate the relationship between HLA-DRB1 alleles and chronic urticaria with positive autologous serum skin test （ASST） in Guangxi Zhuang Autonomous Region.Methods ASST was conducted in 144 patients with chronic urticaria,who were subsequently divided into two groups according to the test result：positive group （n =62） and negative group （n =82）.PCR amplification with sequence-specific primers was used to determine the genotypes of HLA-DRB1 alleles in the patients and 199 normal human controls.Chi-square test was performed to analyse the difference in the frequency of HLA-DRB1 alleles between the 3 groups by using the SPSS 13.0 statistical software package.Results There were significant differences in the frequency of HLA-DRB1＊01,＊1401 and ＊16 alleles among the patients with positive and negative ASST and the controls （x2 =10.92,Pc =0.032;x2 =35.34,Pc 〈 0.01 ;x2 =12.69,Pc =0.032）.Paired comparison revealed significant differences in the frequency of HLA-DRB1＊1401 allele between the patients with positive ASST and controls （RR =17.09,Pc 〈 0.01 ） as well as between the patients with positive and negative ASST （RR =7.20,Pc 〈 0.01）.Conclusion HLA-DRB1＊1401 allele may be,or be linked to,the predisposing gene of chronic urticaria with positive ASST in Guangxi Zhuang Autonomous Region.