犬小孢子菌膜蛋白PQ—LRP基因全长cDNA的克隆

Cloning of full-length cDNA of Microsporum canis membrane protein PQ-loop repeat protein gene

目的 克隆犬小孢子菌膜蛋白PQ-LRP（PQ-loop repeat protein）基因全长cDNA,探讨在头癣发病机制中的作用.方法 选用犬小孢子菌头癣株（A518）为实验株,采用cDNA快速末端扩增法（RACE）,克隆PQ-LRP基因的全长序列.结合生物信息学方法对获得的序列进行初步功能分析.结果 获得犬小孢子菌PQ-LRP全长序列为1522 bp,拥有一个1080 bp的开放阅读框,编码359个氨基酸,5 ＇非编码区为49 bp,3 ＇非编码区为393 bp;同源性比对与断发毛癣菌的PQ-LRP同源性达到81％,与红色毛癣菌PQ-LRP同源性达到79％.结论 克隆出犬小孢子菌膜蛋白PQ-LRP cDNA全长序列,为研究膜蛋白PQ-LRP基因在犬小孢子菌病中的功能奠定基础.

Objective To clone the full-length cDNA of Microsporum canis membrane protein PQ-loop repeat protein （PQ-LRP） gene,so as to investigate the roles of PQ-LRP in the pathogenesis of tinea capitis.Methods A Microsporum canis strain （A518） from a patient with tinea capitis served as the experimental strain.Rapid cDNA end amplification （RACE） was performed to clone the full length cDNA sequence of PQLRP gene.Bioinformatics methods were used to make a preliminary functional analysis of the gene.Results The cDNA of PQ-LRP gene was obtained with a full length of 1522 bp,including the 5＇ untranslated region （49 bp）,coding region （1080 bp） and 3＇ untranslated region （393 bp）.The coding region encoded a protein precursor including 359 amino acid residues.The cloned cDNA of PQ-LRP gene shared an 81% nucleotide identity with that of Trichophyton tonsurans and a 79% nucleotide identity with that of Trichophyton rubrum.Conclusions The full-length cDNA of Microsporum canis membrane protein PQ-LRP gene has been successfully cloned,which will provide an important basis for further researches into the roles of PQ-LRP in Microsporum canis-associated diseases.