兔VX2声门上型喉癌淋巴管生成规律在淋巴转移中的意义

The rule of lymphatic formation in rabbit VX2 supraglottic carcinoma model with lymph node metastasis

目的:建立兔声门上型喉癌动物模型并探讨兔VX2声门上型喉癌淋巴管生成规律。方法:建立VX2声门上型喉癌动物模型,应用VEGFR-3免疫组织化学方法及全自动图像分析仪检测60例兔VX2声门上型喉癌中心区、交界区及正常喉黏膜淋巴管的数密度和面密度,分析其规律。结果:中心区未见到有腔淋巴管的生成,正常喉黏膜仅检测到少量淋巴管,交界区有大量淋巴管生成扩张。交界区与正常黏膜区淋巴管密度相比较差异具有统计学意义(P<0.01)。结论:兔VX2声门上型喉癌淋巴管生成特点模拟了人声门上型喉癌淋巴管生成规律特点,即在交界区有大量淋巴管生成,这与淋巴结转移呈正相关,癌细胞可经此路径转移到区域淋巴结,测定声门上型喉癌淋巴管密度对判定是否发生淋巴结转移具有重要意义,同时这一结论为在动物身上行抑制淋巴管生成的抗肿瘤药物的基础研究提供了理论依据。

Objective：Establishment of transplanted model of VX2 supraglottic carcinoma in rabbits and investigation the rule of lymphatic vessels formation. Method： After establishment of VX2 tumor-bearing rabbits, the carcinoma tissues were transplanted into the operculum laryngis submucosa in sixty New-Zealand white rabbits t.o establish transplanted tumor model. Vascular endothelial growth factor-3 （VEGFR-3） label staining was performed to observe lymphatic vessels. Number density, volume density of lymphatics periphery region of carcinoma, normal region and centre region were measured using computer image analysis system. Result：There was no lymphatic vessels in carcinomatous centre region,but the lymphatic vessels number density, volume density in periphery region was much more than normal region. Their cavities were dilated. The discrepancy had statistical significance（P〈0.01）. Conclusion： The rule of lymphatic formation in rabbit VX2 supraglottic carcinoma model mimesis rule of lymphatic formation anthroposupraglottic carcinoma. Lymphatic multiplication and dilation at periphery region of carcinoma is associated with lymph node metastasis. Evaluation of it at periphery region of carcinoma may be useful in predicting lymph node metastasis in patients with supraglottic carcinoma. This conclusion provides theoretical basis for utility of the antiumor medicines which inhibit lymphatic formation in animal model.