基于漩流的细胞姿态控制方法

Swirl-based Control Method of Cell Orientation

细胞姿态调整是生物工程显微操作中一项非常重要的工作。提出通过两微管喷射流体产生漩流,漩流带动细胞转动,进行非接触式的细胞姿态控制的方法。改变微管之间的相对位置、喷射速度和微管内径等参数,可以控制微管之间产生的漩流强度、尺寸,以适应不同尺寸、形状的细胞以及培养溶液的变化,使显微操作更为方便。建立显微操作环境下微流场漩流中细胞运动模型,模拟微观尺度下利用微流体产生漩流和细胞在漩流中的运动,仿真分析微管相对位置、喷射速度等参数对漩流及细胞运动特性的影响。通过试验进一步研究微流场中漩流的产生以及不同形状漩流作用下细胞的运动特性,得到与数值计算结果相一致的结论。

Cell Orientation adjustment is an important issue in micromanipulation.A non-contact cell orientation method is put forward with which the cell is made to move to desired orientation by the swirl which is produced by microfluid spraying from two tiny tubes.Both the strength and the size of the swirl can be regulated to adapt to different size and shape of cells as well as viscosity of cellular sap by changing the relative position of the tubes,their jetting velocity,their inner diameter and other parameters.This characteristic makes cell micromanipulation more convenient.The model of cell motion in swirl under micromanipulation environment is built up to simulate the swirl generation and cell movement in swirl in micro-scale.The effects of the relative position of tiny tubes and their jetting velocity on the swirl and the cell moving characteristics have been analyzed from simulation results.The experiment is also conducted to discuss the generation of the swirl and cell moving characteristics as far as the swirls in different shapes are concerned and the results show a good agreement with that of numerical simulation.