丹参酮ⅡA对舌鳞状细胞癌细胞系TCA-83增殖和凋亡的影响

Effect of tanshinoneⅡA on the proliferation and apoptosis of tongue cancer cell line TCA-83 in vitro

目的:体外研究丹参酮ⅡA对舌鳞状细胞癌细胞系TCA-83增殖和凋亡的影响。方法:用终浓度为0.625、1.25、2.5、5、10、20 mg/L的丹参酮ⅡA处理TCA-83细胞。应用MTT比色试验,Giemsa染色,Annexin-V-FITC和PI(propidium i-odide)双标记活细胞后,流式细胞仪和透射电镜检测TCA-83细胞在不同时间点的生长抑制率和凋亡情况。结果:丹参酮ⅡA对TCA-83的生长抑制率与药物浓度和作用时间具有明显的正相关性(P<0.01)。用药组细胞凋亡率随处理时间延长和药物浓度增加而上升,各组间差异有显著性(P<0.000 1)。结论:丹参酮ⅡA具有抑制舌癌细胞系TCA-83增殖和诱导其凋亡的作用,且具有浓度和时间依赖性。

Objective： To study the effects of tanshinoneⅡA on the proliferation and apoptosis of tongue cancer cell line TCA-83 in vitro.Methods： TCA-83 cells were treated by tanshinoneⅡA at 0.625,1.25,2.5,5,10 and 20 mg/L respectively.MTT assay,phase contrast microscopy,Giemsa staining,flow cytometry with Annexin-V-FITC/propidium iodide（PI） double staining and transmission electron microscope（TEM） were used to examine the growth inhibition and apoptosis of TCA-83 cells at different time points.Results： The growth inhibition rate of tanshinoneⅡA on TCA-83 cells was positively correlated with concentrations of tanshinoneⅡA and treatment time（P0.01）.The apoptosis of TCA-83 cells was induced by tanshinoneⅡA in a dose-and time-dependant manner（P0.000 1）.Conclusion： TanshinoneⅡA may inhibit the proliferation and induce apoptosis of tongue cancer cell line TCA-83 in time and dose-dependent manner.