摘要
Bamboo shoots grow quickly through the rapid elongation of intemodes. In order to veri~ whether DNA methylation affects bamboo shoot elongation, we compared DNA methylation levels at five develop- mental stages of bamboo （Phyllostachys praecox）, including young bamboo shoot of 40-cm at length （S1）, bamboo shoot of 2-cm at length （S2）, young leaf （Y1）, leaf （Y2）, and aging leaf （M）, by using amplified fragment length polymorphism （AFLP） based on KpnI/MseI and Acc65I/MseI platforms. The polymorphism ratio of methylated to un- methylated DNA sites in bamboo shoot （$1 and $2） was higher than that in leaf （Y1, Y2 and M）. The polymorphism ratio of methylated to un- methylated DNA sites in $2 was highest （32%）. Our results suggest that DNA methylation changed greatly at bamboo growing stages, especially in stage of rapid elongation of intemodes.
Bamboo shoots grow quickly through the rapid elongation of intemodes. In order to veri~ whether DNA methylation affects bamboo shoot elongation, we compared DNA methylation levels at five develop- mental stages of bamboo （Phyllostachys praecox）, including young bamboo shoot of 40-cm at length （S1）, bamboo shoot of 2-cm at length （S2）, young leaf （Y1）, leaf （Y2）, and aging leaf （M）, by using amplified fragment length polymorphism （AFLP） based on KpnI/MseI and Acc65I/MseI platforms. The polymorphism ratio of methylated to un- methylated DNA sites in bamboo shoot （$1 and $2） was higher than that in leaf （Y1, Y2 and M）. The polymorphism ratio of methylated to un- methylated DNA sites in $2 was highest （32%）. Our results suggest that DNA methylation changed greatly at bamboo growing stages, especially in stage of rapid elongation of intemodes.
基金
funded by the Natural Science Foundation of China (30800879)
